溪黄草对肝癌细胞HepG2增殖及凋亡的影响

目的 探讨溪黄草对人肝癌HepG2细胞株增殖和凋亡的影响。方法 MTT法检测溪黄草对肝癌HepG2细胞增殖的抑制作用，流式细胞仪Annexin V/PI 双染法检测对细胞凋亡的影响，ELISA法检测Bcl-2、Bax蛋白表达的情况。结果 不同浓度溪黄草能抑制肝癌细胞HepG2细胞的生长(P＜0.01)，并具有浓度和时间依赖性。0.75g/μL的溪黄草作用于HepG2细胞3、6h后，结果表明溪黄草能显著促进细胞凋亡，具有时间依赖性，与阴性对照组比较有统计学差异(P＜0.05)。0.75g/μL溪黄草作用HepG2 细胞6h后，Bcl-2蛋白表达降低，Bax蛋白表达增加，与阴性对照组比较，亦具有统计学差异(P＜0.05)。结论 溪黄草能抑制HepG2细胞的体外增殖，且抑制作用表现出时效关系，其机制可能与促进细胞凋亡、下调Bcl-2蛋白及上调Bax蛋白表达有关。

Effect of Rabdosia lophanthoides on the proliferation and apoptosis in hepatoma carcinoma cell line HepG2

Objective To investigate the effect of Rabdosia lophanthoides on the proliferation and apoptosis in hepatoma carcinoma cell line HepG2. Methods The effect of Rabdosia lophanthoides on the cell proliferation and cell apoptosis was detected by the MTT method and flow cytometry.The expressions of Bax and Bcl-2 protein were detected by the ELISA method. Results After incubation with different concentrations of Rabdosia lophanthoides, the growth of hepatoma carcinoma cell line HepG2 was significantly inhibited in a time- and dose-dependent manner(P<0.01). After the cells were treated with 0.75g/μL Rabdosia lophanthoides for 3 and 6 h, the apoptosis was induced. The apoptosis of hepatoma carcinoma cell line HepG2 was significantly inhibited in a time-dependent manner. Compared with the negative control group, the cell apoptosis was induced significantly (P<0.05). After treated with 0.75g/μL Rabdosia lophanthoides for 6h, the expression of Bcl-2 protein decreased, and the expression of Bax protein increased.The difference had statistical significance (P<0.05). Conclusion Rabdosia lophanthoides can inhibit cell proliferation of hepatoma carcinoma cell line HepG2 in a time- and dose-dependent manner. The mechanism might relate to inducing cell apoptosis, decreasing the expression of Bcl-2 and increasing the expression of Bax.