人脐带间充质干细胞移植治疗大鼠急性肺损伤

背景：间充质干细胞具有免疫调节特性,脐带间充质干细胞因其特有的优势,将在急性肺损伤和急性呼吸窘迫综合征的临床应用中有着光明的前景。目的：探讨脐带间充质干细胞移植对内毒素性大鼠急性肺损伤模型的保护作用。方法：将48只健康雄性SD大鼠随机均分为正常对照组、急性肺损伤组和脐带间充质干细胞移植组。后两组采用经气管内滴注内毒素建立急性肺损伤模型。成功造模1 h后,脐带间充质干细胞移植组,经气管内滴注脐带间充质干细胞混悬液,正常对照组和急性肺损伤组同法予以等量生理盐水。分别在干预后24,72 h,观察肺组织病理改变,检测病理组织评分、肺组织干湿质量比、髓过氧化物酶活性及血浆白细胞介素6、白细胞介素10及肿瘤坏死因子α水平。 结果与结论：脐带间充质干细胞移植可以减轻内毒素诱导的急性肺损伤模型的损伤程度;脐带间充质干细胞移植组在各时间点与急性肺损伤组比较,病理损伤评分、肺干湿质量比、肺组织髓过氧化物酶活性、血浆白细胞介素6和肿瘤坏死因子α水平均明显降低,血浆白细胞介素10水平明显升高。说明脐带间充质干细胞对内毒素性急性肺损伤模型有保护作用;其保护机制可能为脐带间充质干细胞移植维持肺内炎性递质和抗炎递质平衡。

Human umbilical cord-derived mesenchymal stem cell transplantation for the treatment of acute lung injury in rats

BACKGROUND:Mesenchymal stem cells have immunomodulatory properties. Umbilical cord-derived mesenchymal stem cells have unique advantages, and have a bright future in the clinical treatment of acute lung injury and acute respiratory distress syndrome.OBJECTIVE:To investigate the protective effect of umbilical cord-derived mesenchymal stem cell transplantation on endotoxin-induced acute lung injury model.METHODS:Forty-eight healthy male Sprague Dawley rats were randomly divided into normal control group, acute lung injury group and umbilical cord-derived mesenchymal stem cell transplantation group. The rats in the acute lung injury group and umbilical cord-derived mesenchymal stem cell transplantation group were used to establish the acute lung injury model through intratracheal instillation of endotoxin. One hour after modeling, rats in the umbilical cord-derived mesenchymal stem cell transplantation group were instilled with umbilical cord-derived mesenchymal stem cells suspension intratracheally, and the rats in the normal control group and acute lung injury group were instilled with normal saline in the same dose intratracheally. After treated for 24 and 72 hours, the pathological changes of the lung tissue were observed, and the histological score, wet and dry weight ratio of the lung tissue, myeloperoxidase activity and the levels of interleukin-6, interleukin-10 and tumor necrosis factor-α were detected.RESULTS AND CONCLUSION:Umbilical cord-derived mesenchymal stem cell transplantation could attenuate lung injuries in rat models of acute lung injury. Compared with acute lung injury group, the histological score, wet and dry weight ratio of the lung tissue, myeloperoxidase activity and the levels of interleukin-6 and tumor necrosis factor-α in the umbilical cord-derived mesenchymal stem cell transplantation group were decreased at different time points, while the level of interleukin-10 was increased. Umbilical cord-derived mesenchymal stem cells have protective effect on endotoxin-induced acute lung injury models; the protection mechanisms may be that the umbilical cord-derived mesenchymal stem cell transplantation can maintain the balance between inflammatory mediators and anti-inflammatory mediators in lung tissues.