不同矫治正畸作用下牙周骨改建过程中压力侧相关因子的变化

背景：近年来研究表明核因子κB受体活化因子配体与正畸牙移动骨改建过程中破骨细胞的形成、分化和功能密切相关。 目的：观察不同矫治器正畸作用下兔牙周组织改建过程中压力侧组织核因子κB受体活化因子配体表达的变化,探讨不同矫治器的正畸效果。 方法：将64只健康大耳白兔随机分为4组：对照组、MBT矫治器组、Begg矫治器组、Damon Ⅲ矫治器组,每组16只。MBT矫治器组、Begg矫治器组、Damon Ⅲ矫治器组分别应用对应的矫治器对兔上颌切牙和第一磨牙进行矫治,近中移动牵引力为80 g;对照组不进行矫治。分别于矫治后第3,7,14,21天每组取4只白兔进行检测。 结果与结论：苏木精-伊红染色显示加力后各矫治器组压力侧牙周膜腔变窄,牙槽骨边缘出现骨吸收陷窝。抗酒石酸酸性磷酸酶染色显示,矫治7 d时,骨改建活跃,破骨细胞数量达到高峰;同时实时荧光定量PCR检测发现加力后压力侧牙槽骨组织中核因子κB受体活化因子配体mRNA的表达明显增高,并于加力后第7天达到最高峰,而后逐渐降低。加力第7天时,Damon Ⅲ矫治器组破骨细胞数量和核因子κB受体活化因子配体mRNA表达水平均明显高于MBT矫治器组和Begg矫治器组(P < 0.05)。提示在骨改建过程中核因子κB受体活化因子配体mRNA表达的变化规律与破骨细胞数量相一致,Damon Ⅲ矫治器的矫治效果优于MBT矫治器、Begg矫治器。

Changes of related factors on the pressure side under different appliance orthodontic during periodontal bone remodeling

BACKGROUND:Recent studies have shown that receptor activator of nuclear factor-κB ligand is closely related to osteoclast differentiation, formation and function in bone remodeling during orthodontic tooth movement. OBJECTIVE:To observe the changes in receptor activator of nuclear factor-κB ligand expression in the  pressure side during periodontal tissue remodeling under different appliance orthodontics, and to explore the effect of different orthodontic appliances.  METHODS:Sixty-four healthy rabbits were divided into four groups: control group, MBT appliance group, Begg appliance group, Damon Ⅲ appliance group, 16 rabbits in each group. In the MBT appliance group, Begg appliance group and Damon Ⅲ appliance group, corresponding appliances were used to correct the maxillary incisors and first molars respectively, and the mesial movement traction force was 80 g; the control group did not receive correction. Four rabbits were selected from each group for testing at 3, 7, 14 and 21 days after correction. RESULTS AND CONCLUSION:Hematoxylin-eosin staining showed that the periodontal ligament cavity in the pressure side was narrow and the edge of the alveolar bone exhibited resorption pits. Tartrate-resistant acid phosphatase staining showed bone remodeling was active and the number of osteoclasts reached peak at 7 days after correction. Real-time quantitative PCR showed that the mRNA expression of receptor activator of nuclear factor-κB ligand on the pressure side of the alveolar bone tissue was significantly increased after force loading, and reached peak at 7 days after forcing, then gradually decreased. At 7 days after forcing, the number of osteoclasts and the mRNA expression of receptor activator of nuclear factor-κB ligand in the Damon Ⅲ appliance group were higher than those in the MBT appliance group and Begg appliance group (P < 0.05). In bone remodeling process, mRNA expression of receptor activator of nuclear factor-κB ligand was positively correlated with the number of osteoclasts, and the effect of Damon Ⅲ appliance was better than MBT and Begg appliances.