自体骨髓间充质干细胞移植梗死心肌猪的心功能变化

背景：干细胞移植可改善缺血性心肌血供并改善心功能。 目的：进一步验证自体骨髓间充质干细胞在心肌梗死后的应用及效果评价。 方法：15只健康太湖梅山猪通过冠脉栓塞建立心肌梗死模型。随机分为3组，每组5只，其中2组分别在心肌梗死后3 h，2周行自体骨髓干细胞移植，模型组不移植细胞。行心脏超声观察心脏功能各指标的改变；并在不同时间点检测血清血管内皮生长因子值；在实验终点取大体标本并通过免疫组织化学检测移植细胞在心肌内定植及分化情况，检测心肌血管密度。 结果与结论：心肌梗死3 h组与模型组比较，射血分数、左室舒张期内径、左室收缩期内径各项心功能指标及心肌血管密度、不同时间点血清血管内皮生长因子水平差异无显著性意义。心肌梗死后2周移植组与模型组相比，心功能指标均有改善，心肌血管密度大于模型组，血清血管内皮生长因子水平明显高于较移植前(P < 0.05)。提示不同时间点心肌微环境对于骨髓来源的间充质干细胞分化及定植的影响，心肌梗死后急性期内局部微环境不利于移植细胞的存活，在瘢痕修复早期行骨髓干细胞移植对骨髓干细胞的分化和定植以及对心功能的改善有较好的效果。

Cardiac function of pigs with myocardial infarction after autologous bone marrow mesenchymal stem cell transplantation

BACKGROUND:Stem cells transplantation can improve the ischemic myocardial blood supply and improve the cardiac function. OBJECTIVE:To further identify the application and effect of autologous bone marrow mesenchymal stem cells on the cardiac function after myocardial infarction. METHODS:Fifteen Taihu Meishan pigs were selected to make the myocardial infarction models, and then divided into four groups (5 swine in each group). At 2 weeks after modeling, the pigs were treated with autologous bone marrow stem cells transplantation. The change in each index of cardiac function was observed with Doppler. The level of serum vascular endothelial growth factor was detected in different periods after transplantation. At the end of the experiment, the general specimens were removed, and the colonization and differentiation of the transplanted cells were detected with immunohistochemistry, and the myocardial vascular density was also detected. RESULTS AND CONCLUSION:There were no significant differences in ejection fraction, left ventricular internal dimension diastole and left ventricular internal dimension systole, as well as the myocardial vascular density and the level of serum vascular endothelial growth factor at different time points between myocardial infarction 3 hours group and model group. Compared with the model group, the cardiac function indicators were improved in the transplantation group at 2 weeks after transplantation, the myocardial vascular density in the transplantation group was higher than that in the model group, the cardiac function at 2 weeks after myocardial infarction was better than others and the serum vascular endothelial growth factor level was improved after transplantation (P < 0.05). The results suggest that myocardial microenvironment at different time points has different effects on bone marrow mesenchymal stem cell differentiation and colonization, and bone marrow-derived stem cell transplantation in the early scar repairing has positive effects on the improvement of cardiac function and the differentiation and colonization of bone marrow-derived stem cells.