富血小板血浆对颗粒脂肪移植的影响

背景：较高的远期吸收率极大地影响了脂肪移植在临床的广泛应用。富血小板血浆中含有丰富的促进组织修复与再生的生长因子。 目的：观察富血小板血浆对颗粒脂肪移植效果的影响，并初步探索其作用机制。 方法：将10只6周龄的裸鼠随机选择左侧或右侧背部皮下组织作为富血小板血浆组(0.5 mL人颗粒脂肪+0.1 mL富血小板血浆)受区，另外一侧作为对照组(0.5 mL人颗粒脂肪+0.1 mL磷酸盐缓冲液)受区。于植入后10，90 d各选择5只裸鼠，处死后取出移植物进行大体形态观察、体积测定及组织学检测。此外，体外实验中，从人离体脂肪组织中分离培养脂肪干细胞，分别通过Cell Counting Kit-8和Real Time-PCR检测富血小板血浆对脂肪干细胞体外增殖和成脂分化的影响。 结果与结论：比较植入后10，90 d所得的移植物发现，富血小板血浆组存活体积明显多于对照组(P < 0.05)，组织学上也含有更多的正常脂肪细胞(P < 0.05)及微血管数目(P < 0.05)。体外实验中，富血小板血浆显著促进了脂肪干细胞的增殖，同时在其诱导的脂肪干细胞中检测到成脂分化相关基因表达升高。说明富血小板血浆能够提高脂肪移植的成活，这可能与富血小板血浆促进脂肪干细胞的增殖及成脂分化以及促进移植脂肪组织的血管化密切相关。

Platelet-rich plasma effects on grainy fat transplantation

BACKGROUND:The higher long-term absorption rate greatly influence the widely application of fat transplantation. Platelet-rich plasma contains a high concentration of growth factors, which benefits to the tissue healing and regeneration. OBJECTIVE: To observe the effects of platelet-rich plasma on grainy fat transplantation and to investigate the mechanisms preliminarily. METHODS: Ten 6-week-old nude mice were prepared. The right or left dorsal subcutaneous tissues were randomly selected as the platelet-rich plasma group (0.5 mL fat granule+0.1 mL platelet-rich plasma), and the contralateral side was regarded as the control group (0.5 mL fat granule+0.1 mL phosphate-buffered saline). At 10 and 90 days after implantation, five nude mice were selected from each group, and then the mice were sacrificed to obtain the grafts in each group for general appearance observation, volume determination and histological detection. Furthermore, we isolated adipose-derived stem cells from human subcutaneous fat tissue during the in vitro experiment. Cell counting kit-8 and real-time PCR were used to evaluate the influence of platelet-rich plasma on adipose-derived stem cell proliferation and adipogenic differentiation in vitro, respectively. RESULTS AND CONCLUSION:Comparison of the grafts obtained at 10 and 90 days after implantation, the residual volume in the platelet-rich plasma group was significantly larger than that in the control group (P < 0.05), Moreover, more normal adipocytes and capillary formation were observed in the platelet-rich plasma group (P < 0.05). For in vitro experiment, platelet-rich plasma could significantly improve adipose-derived stem cell proliferation, and the expressions of adipogenic-related genes were up-regulated in platelet-rich plasma-induced adipose-derived stem cells. All results demonstrate that platelet-rich plasma can improve the survival of fat grafts, which might be closely related to that platelet-rich plasma can promote the proliferation and adipogenic differentiation of adipose-derived stem cells and the revascularization in grafted fat tissue.