磁性复合骨水泥的体外细胞毒性 |
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作者姓名: | 唐正海 王晓文 张友仁 潘 琳 唐劲天 岳秉飞 |
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作者单位: | 1北京中医药大学,北京市 100102
2清华大学玉泉医院脑神经疾病研究所,北京市 100049
3中日友好医院临床医学研究所,北京市 100029
4中国药品生物制品检定所,北京市 100050 |
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基金项目: | 国家自然科学基金项目(81071885) |
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摘 要: | 背景:磁性复合骨水泥作为一种新型骨转移治疗材料,不仅能对骨缺损进行修复,还能在交变磁场下升温杀死骨肿瘤。
目的:检测磁性复合骨水泥对小鼠L929成纤维细胞增殖活性的影响及细胞毒性反应。
方法:采用CCK-8法检测含0%(即磷酸钙骨水泥组),10%,20% Fe3O4的磁性磷酸钙骨水泥浸提液及含0%(即聚甲基丙烯酸甲酯骨水泥组),10%,20%Fe3O4的聚甲基丙烯酸甲酯骨水泥浸提液对对数生长期小鼠L929成纤维细胞的毒性作用,每种浸提液又分为50%与100%两个浓度梯度;以正常培养基培养的小鼠L929成纤维细胞作为阴性对照组。
结果与结论:①在100%浓度梯度下:除含20%Fe3O4的磁性聚甲基丙烯酸甲酯骨水泥组细胞数量较少、细胞形态异常外,其余磁性聚甲基丙烯酸甲酯骨水泥组细胞生长状态良好;含10%Fe3O4的磁性磷酸钙骨水泥组培养24 h时表现出轻度细胞毒性,毒性2级;含20%Fe3O4的聚甲基丙烯酸甲酯骨水泥在24- 72 h为表现出轻、中度细胞毒性,毒性为2,3级。各磁性磷酸钙骨水泥组细胞数量及形态正常。②在50%浓度梯度下:各组细胞形态均正常,细胞毒性为0级或1级。表明磁性聚甲基丙烯酸甲酯骨水泥具有轻、中度细胞毒性,磁性磷酸钙骨水泥细胞相容性良好,基本无细胞毒性。
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关 键 词: | 生物材料 组织工程骨材料 磁性骨水泥 聚甲基丙烯酸甲酯 磷酸钙 生物相容性 成纤维细胞 细胞毒性 增殖 国家自然科学基金 |
收稿时间: | 2012-08-13 |
Cytotoxicity of the magnetic bone cement in vitro |
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Authors: | Tang Zheng-hai Wang Xiao-wen Zhang You-ren Pan Lin Tang Jin-tian Yue Bing-fei |
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Institution: | 1 Beijing University of Chinese Medicine, Beijing 100102, China
2 Institute of Neurological Disorders, Yuquan Hospital, Tsinghua University, Beijing 100049, China
3 Institute of Clinical Medical Science, China-Japan Friendship Hospital, Beijing 100029, China
4 National Institutes for Food and Drug Control, Beijing 100050, China |
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Abstract: | BACKGROUND: Magnetic bone cement has been used as a new kind of material for bone metastasis, and it can repair bone defects and cure bone tumors with temperature rising under an alternating magnetic field.
OBJECTIVE: To test the magnetic bone cement effects on cytotoxicity and cell proliferation of mouse L929 cells.
METHODS: The cytotoxicity of magnetic calcium phosphate cement and polymethylmethacrylate bone cement leaching liquors, respectively, containing 0, 10%, 20% Fe3O4, to L929 cells at logarithmic growth phase was detected using Cell Count Kit-8 assay. Two kinds of leaching liquors were prepared to two concentration gradients, 50% and 100%. Mouse L929 cells cultured in normal culture medium were considered as control group.
RESULTS AND CONCLUSION: (1) Under 100% concentration gradient: Cells grew well in polymethyl methacrylate bone cement groups containing 0, 10% Fe3O4 except for 20% Fe3O4. Magnetic calcium phosphate cement containing 10% Fe3O4 showed mild cell toxicity, graded as 2, after culture for 24 hours. During 24- 72 hours, polymethylmethacrylate bone cement containing 20% Fe3O4 exhibited mild or moderate cell toxicity and the toxicity was graded as 2 or 3. (2) Under 50 concentration gradient: Cells grew well in all groups. The cell toxicity was graded as 0 or 1. Magnetic polymethylmethacrylate bone cement has mild or moderate cell toxicity and magnetic calcium phosphate cement exhibits a good biocompatibility with no cell toxicity. |
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Keywords: | biomaterials tissue-engineered bone materials magnetic bone cement polymethylmethacrylate calcium phosphate biocompatibility fibroblasts cytotoxicity proliferation National Natural Science Foundation of China |
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