胎盘间充质干细胞的体外诱导分化

背景：与骨髓、外周血、脂肪及胚胎等来源的间充质干细胞相比,脐带和胎盘组织来源的间充质干细胞具有来源广泛、易于获得、不引起供者不适以及不存在道德伦理争议等优势。目的：探讨胎盘间充质干细胞体外分离、培养、传代的方法及其分化潜能,为胎盘间充质干细胞的临床应用提供基础研究依据。方法：应用计算机检索2000年1月至2011年12月PubMed数据库、中国期刊全文数据库、万方数据库与胎盘间充质干细胞有关的文章,中文检索词为“胎盘间充质干细胞,体外,诱导分化”,英文检索词为“Placenta-derived mesenchymal stem cells,Induction and differentiation in vitro”。共检索到文献98篇,最终纳入符合标准的33篇文献。结果与结论：目前国内胎盘间充质干细胞还处于基础研究阶段,国外胎盘间充质干细胞在实验研究方面已经取得了一定的进展,而且也在临床应用研究方面有了一定的突破。胎盘间充质干细胞具有来源广泛,取材方便,不引起供者不适以及不涉及伦理道德问题,与捐献骨髓或采集动员外周血相比,供者无痛苦,感染机会少, 扩增能力强等,在临床治疗方面有待于进一步深入研究。

In vitro induced differentiation of placenta-derived mesenchymal stem cells

BACKGROUND:Compared to bone marrow-, peripheral blood-, fat- and embryo-derived mesenchymal stem cells, umbilical cord- and placenta-derived mesenchymal stem cells possess advantages including wide source, ease of harvesting, no immunological rejection or ethical compromise.OBJECTIVE:To investigate the methods to in vitro isolate, culture and sub-culture of placenta-derived mesenchymal stem cells, providing experimental basis for clinical application of placenta-derived mesenchymal stem cells.METHODS:A computer-based online retrieval was performed to search papers regarding placenta-derived mesenchymal stem cells published in PubMed database, Chinese Journal Full Text Database, Wanfang database using key words “placenta-derived mesenchymal stem cells, induction and differentiation in vitro”. A total of 98 papers were retrieved, and 33 were suitable for final analysis.RESULTS AND CONCLUSION:Placenta-derived mesenchymal stem cells are in the stage of basic research in China, but some progress has been made in basic research and clinical application of placenta-derived mesenchymal stem cells in countries outside China. Placenta-derived mesenchymal stem cells possess advantages including wide source, ease in sample harvesting and no immunological rejection or ethical compromise as well as less pain, fewer infection opportunities and stronger proliferative capacity compared to bone marrow donation or peripheral blood collection. For these reasons, placenta-derived mesenchymal stem cells should be further studied.