梯度掺锶羟基磷灰石骨水泥的体外细胞生物学性能

背景：锶掺入羟基磷灰石骨水泥后可以改善材料的结晶性和相容性，但产生的细胞毒性以及对细胞表面黏附、增殖和表达的影响还需要深入研究。目的：观察梯度掺锶羟基磷灰石骨水泥的体外细胞生物学性能。方法：制备4组羟基磷灰石骨水泥试样，分别编为0%，1%，5%和10%掺锶羟基磷灰石骨水泥，每组取6个平行试样紫外线照射灭菌3 h备用。浸提递质为含体积分数10%胎牛血清的DMEM培养液。按照掺锶羟基磷灰石骨水泥粉末质量∶浸提液=1 g∶10 mL比例配制。选用L-929成纤维细胞和幼兔成骨细胞，对成骨细胞进行鉴定。扫描电镜观察幼兔成骨细胞在梯度掺锶羟基磷灰石骨水泥表面的黏附和增殖情况，细胞的表达情况采用碱性磷酸酶活性检测法检测。结果与结论：①不同掺锶量羟基磷灰石骨水泥的细胞毒性评级均为0或1级，各试样的细胞毒性与其掺锶量、作用时间、浸提液浓度有一定关联性。②适量锶元素的加入可以促进成骨细胞的黏附、增殖及表达，改善材料的溶解动力学，提高其生物降解性，更加符合临床要求。但目前尚缺乏有关的远期实验结果。

In vitro biological properties of gradient strontium-containing hydroxyapatite bone cement

BACKGROUND:Strontium-containing hydroxyapatite bone cement can improve the crystallinity and compatibility of the materials, but the effects on cell toxicity, cell surface adhesion, proliferation and expression still need further studies.OBJECTIVE:To observe the in vitro cytobiological properties of gradient strontium-containing hydroxyapatite bone cement.METHODS:Four groups of strontium-containing hydroxyapatite bone cement samples were prepared at a strontium content of 0%, 1%, 5% and 10%. Six parallel samples selected from each group were subjected to ultraviolet radiation sterilization for 3 hours for standby. Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum served as extraction neurotransmitter. Strontium-containing hydroxyapatite cement powder was mixed with extract solution at a ratio of 1 g to 10 mL. L-929 fibroblasts and osteoblasts from immature rabbits were selected and identified. Scanning electron microscopy was used to observe the adhesion and proliferation of osteoblasts on the surface of gradient strontium-containing hydroxyapatite cement, and cell viability was detected using alkaline phosphatase activity method.RESULTS AND CONCLUSION:(1) Cytotoxicity of different amount of strontium-containing hydroxyapatite cement was graded 0 or 1, suggesting the cytotoxicity of each sample was certainly associated with strontium  content, reaction time, and extract concentration. (2) Hydroxyapatite bone cement containing a certain amount of strontium can promote osteoblast adhesion, proliferation and expression and improve the dissolution kinetics and biodegradability of materials, becoming more in line with the clinical requirements. But there is still lack of long-term results.