人工脑膜复合大鼠骨髓间充质干细胞修复心肌梗死

背景：干细胞移植治疗心肌梗死拥有广泛的应用前景,寻求理想的细胞类型和有效的移植方式是提高干细胞治疗效果的关键因素。 目的：探讨人工脑膜复合骨髓间充质干细胞修复心肌梗死的安全性及作用。 方法：采用全骨髓贴壁筛选法分离培养骨髓间充质干细胞,取培养良好的第3代骨髓间充质干细胞经DAPI标记后接种于人工脑膜制备细胞人工脑膜复合物。构建SD大鼠心肌梗死模型,60只大鼠随机数字表法均分为假手术组、心肌梗死组、人工脑膜组、细胞脑膜复合物组。移植4 周后检测心功能参数,Western blot检测心肌组织缝隙连接蛋白43的表达,计算心肌梗死后生存率。 结果与结论：构建心肌梗死模型并移植后4周,细胞脑膜复合物组心脏组织冰冻切片于荧光显微镜下可观察到心肌内少量核蓝染的细胞,表明骨髓间充质干细胞得以存活;细胞脑膜复合物组与心肌梗死组和人工脑膜组相比,左心室功能明显改善,Cx43蛋白的表达上调,生存率增加(P < 0.05)。说明人工脑膜复合骨髓间充质干细胞移植可提高心肌梗死大鼠心脏功能及生存率。

Bone marrow mesenchymal stem cells cultured in artificial meninges repair infarcted myocardium in rats

BACKGROUND:Stem cell transplantation has broad application prospects in the treatment of myocardial infarction. To find the ideal cell type and the effective transplantation method is the key factor to improve. OBJECTIVE:To investigate the effect and safety of artificial meninges combined with bone marrow mesenchymal stem cells on repairing infarcted heart. METHODS:Bone marrow mesenchymal stem cells were obtained by whole bone marrow culture method, and subcultured to the third generation. Then bone marrow mesenchymal stem cells were labeled with 2-(4-amidinophenyl)- 6-indolecarbamidine dihydrochloride and seeded on the cells and artificial meningeal complex. Sprague Dawley rats model of myocardial infarction were established, and 60 rats were randomly divided into sham-operation group, myocardial infarction group, artificial meningeal group, and cells and artificial meningeal complex group. The cardiac function parameters were tested at 4 weeks after transplantation, Western blot was used to test the expression of myocardial tissue connexin 43, and the survival rate was calculated after myocardial infarction. RESULTS AND CONCLUSION:Frozen sections of the heart tissues showed a few blue-stained cells under the fluoroscope in the complex group at 4 weeks after myocardial infarction and transplantation, which indicated that a few bone marrow mesenchymal stem cells survived in the heart. Compared with myocardial infarction group and artificial meningeal group, left ventricular function was improved, the expression of connexin 43 protein and the survival rate were increased in the complex group (P < 0.05). Artificial meninges combined with bone marrow mesenchymal stem cells can improve cardiac function and survival rate in myocardial infarction rats.