脂肪间充质干细胞复合血小板凝胶修复兔椎间盘退变

背景：富含血小板的血浆凝胶作为三维支架使其中干细胞可以呈立体生长,同时富含血小板的血浆凝胶又释放大量生长因子,促进脂肪间充质干细胞增殖及分化。 目的：探讨脂肪间充质干细胞-富含血小板的血浆凝胶复合体注入兔椎间盘退变模型后的修复作用。 方法：取兔动脉血采用二次离心法制备自体富血小板血浆,取兔肩胛间区脂肪分离培养脂肪间充质干细胞,制备脂肪间充质干细胞-富含血小板的血浆凝胶复合体。新西兰大白兔随机分为对照组、模型组、富含血小板的血浆凝胶组和脂肪间充质干细胞-富含血小板的血浆凝胶复合体组,后3组以穿刺法制备椎间盘退变模型,退变模型制备完成2周后,富含血小板的血浆凝胶组和脂肪间充质干细胞-富含血小板的血浆凝胶复合体组分别对退变间盘中注射相应材料。 结果与结论：兔椎间盘退变后,间隙明显降低,髓核信号明显降低,髓核内基质高,密度染色较深;而经富含血小板的血浆凝胶和脂肪间充质干细胞-富含血小板的血浆凝胶复合体治疗后,上述症状明显改善,且脂肪间充质干细胞-富含血小板的血浆凝胶复合体的治疗效果更好。提示对退变椎间盘内注射富含血小板的血浆凝胶支架及脂肪间充质干细胞-富含血小板的血浆凝胶复合体均有利于减少退变对椎间盘的影响,其中脂肪间充质干细胞-富含血小板的血浆凝胶复合体注射效果更为突出。

Adipose-derived mesenchymal stem cells combined with platelet gel for repair of intervertebral disc degeneration in rats

BACKGROUND: Platelet-rich plasma gel serves as a three-dimensional scaffold in which stem cells can exhibit a three-dimensional growth. Meanwhile, platelet-rich plasma gel can release large amounts of growth factors to promote the proliferation and differentiation of adipose-derived mesenchymal stem cells. OBJECTIVE: To explore the repair effect of injection of autologous platelet rich plasma-adipose derived mesenchymal stem cells complex on rabbit intervertebral disc degeneration. METHODS: Rabbit arterial blood was extracted to prepare autologous platelet-rich plasma using secondary centrifugal method, and adipose-derived mesenchymal stem cells were isolated from the fat tissue of rabbit scapular area. Then, adipose-derived mesenchymal stem cells-platelet-rich plasma gel complex was prepared. New Zealand white rabbits were randomized into control group, model group, platelet-rich plasma gel group and complex group (adipose-derived mesenchymal stem cells-platelet-rich plasma gel complex). Intervertebral disc degeneration model was made in the latter three groups using puncture method. At 2 weeks after modeling, corresponding materials were injected into the disc in the four groups, respectively. RESULTS AND CONCLUSION: After intervertebral disc degeneration in rabbits, the intervertebral gap was significantly reduced, the nucleus pulposus signal was obviously decreased, nucleus pulposus matrix appeared to have a high density and to be deeply stained. The symptoms above mentioned were improved significantly after intervention with the platelet-rich plasma gel or the complex of adipose-derived mesenchymal stem cells-platelet-rich plasma gel, while the latter was better in curative effects. Injection of platelet-rich plasma gel and adipose-derived mesenchymal stem cells-platelet-rich plasma gel complex can reduce the influence of disc degeneration, and the effect of adipose-derived mesenchymal stem cells-platelet-rich plasma gel complex is more outstanding.