大豆异黄酮酊干预Ⅱ度烫伤模型小鼠创面的愈合

背景：雌激素具有促进创面愈合的作用,大豆异黄酮能与雌激素受体结合,具有明显的雌激素样效应。目的：探索大豆异黄酮酊的制备、观察其对小鼠烫伤创面愈合的影响。方法：用冷浸渍法制备大豆异黄酮酊,筛选最佳有效浓度。用昆明小鼠造Ⅱ度烫伤模型,随机分为6组。药物组分别应用0.100,0.361,1.000,3.610 g/L的大豆异黄酮酊局部涂抹;溶剂对照组应用体积分数75%乙醇局部涂抹;空白对照组不作任何处理。从第 3 天起,隔天记录创面面积、计算未愈合创面占原始创面的百分比,于造模后第 3,9,14 天取创面组织做病理切片,观察形态学改变和创面愈合情况。结果与结论：大豆异黄酮酊的最佳效应浓度是3.61 g/mL。在创伤后第 5,7,9,11,13 天,大豆异黄酮酊组的未愈合创面占原始创面的百分比明显小于溶剂对照组和空白组(P < 0.05)。病理组织切片显示,第 9 天,大豆异黄酮酊组的成纤维细胞明显多于溶剂对照组和空白组,第 14 天,大豆异黄酮酊组的表皮生长情况比溶剂对照组和空白组好。表明以75%乙醇为溶剂制备大豆异黄酮酊是可行的;  3.61 g/L的大豆异黄酮酊对小鼠创面的愈合具有明显的促进用。

Influence of soy isoflavone tincture on wound healing of deep partial-thickness scald in mice

BACKGROUND:Estrogen has a role to promote wound healing, and combination of soy isoflavones and estrogen receptor presents an obvious estrogen-like effect.OBJECTIVE:To explore the preparation of soy isoflavone tincture and to study its influence on wound healing in scalded mice. METHODS :Soy isoflavone tincture was prepared by cold dipping and the most effective concentration wasscreened. At the beginning of the experiment, all of the mice were used to prepare deep partial-thickness scald models and then randomly divided into soy isoflavone tincture (0.100, 0.361, 1.000, 3.610 g/L), solvent control (75% ethanol) and blank (without treatment) groups. From the 3rd day on, nonhealing rates of wound were assessed every other day. Simultaneously, the histological and morphological changes were observed and analyzed at days 3, 9, 14 after modeling.RESULTS AND CONCLUSION:The most effective concentration of soybean isoflavone tincture was 3.61 g/L. On days 5, 7, 9, 11, 13 after injury, the nonhealing rate of wound in the soybean isoflavone tincture group was significantly less than that of the blank group (P < 0.05), as well as that of the solvent control group (P < 0.05). On day 9 post scalded, the histomorphology displayed that there were many more fibroblasts in the granulation tissue in the soy isoflavone tincture group than those of the blank group, also than those of the solvent control group. On day 14 post scalded, the histomorphology showed that the epithelial layer in the soy isoflavone tincture group was thicker than that of the other two groups. It is a viable method that soy isoflavone tincture is prepared in 75% ethanol solution, and its concentration of 3.61 g/L can significantly accelerate the wound healing in scalded mice by local application.