微胶珠化胰岛肌肉内移植治疗小鼠1型糖尿病

背景：前期实验证明海藻酸钡微胶珠具有免疫隔离作用,并且不会引起免疫排斥反应。目的：观察包裹胰岛海藻酸钡微胶珠对1型糖尿病小鼠的治疗作用。方法：分离纯化SD大鼠胰腺单个胰岛细胞团,并包裹于海藻酸钡微胶珠内。以腹腔注射链脲佐菌素诱导建立C57BL/6小鼠1型糖尿病模型,随机分组：实验组小鼠股二头肌内多点注射包裹胰岛的海藻酸钡微胶珠,对照组小鼠股二头肌内多点注射胰岛,糖尿病对照组及正常对照组小鼠股二头肌内多点注射生理盐水。术后观察小鼠血糖及胰岛微胶珠在肌肉内存在状况。结果与结论：分离纯化后获得高纯度胰岛,每只供体可获得(905.4±34.5)个,并具有良好生物活性。实验组小鼠血糖降为正常的时间约为6.3 d,移植胰岛存活时间大于30 d,对照组小鼠血糖一直未降至正常,移植胰岛存活时间约为4 d。实验组降糖速率明显快于对照组和糖尿病对照组(P < 0.05)。表明包裹胰岛的海藻酸钡微胶珠镶嵌在肌肉组织中可良好存活,治疗小鼠1型糖尿病。

Intramuscular transplantation of macroencapsulated islets for type 1 diabetes mellitus in mice

BACKGROUND:Alginate-barium chloride microbeads confer immune isolation and prevent graft rejection in preliminary experiments.OBJECTIVE:To assess the role of alginate- barium chloride microbead encapsulation in the treatment of type 1 diabetes mellitus in mice.METHODS:The single islet cell group was isolated from the pancreas of Sprague-Dawley rats and purified. And cells were encapsulated into alginate-barium chloride microbeads. Type 1 diabetes mellitus was induced with streptozotocin into C57BL/6 mice via intraperitoneal injection, and randomly divided into four groups. The pure islets encapsulated in alginate-barium chloride microbeads were transplanted into the biceps femoris in the mice with type 1 diabetes mellitus, as experimental group. The pure islets were transplanted into diabetic recipients at biceps femoris, as control group. Normal C57BL/6 mice were injected with saline into biceps femoris, as normal control group. Diabetic C57BL/6 mice were injected with saline into biceps femoris, as diabetes control group. Then the blood glucose levels and macroencapsulated islets at the transplantation site were observed.RESULTS AND CONCLUSION:After isolation and purification, (905.4±34.5) islets per mouse were obtained and possessed excellent activity. Duration of normal blood glucose after operation in experimental group was approximately 6.3 days. The islet graft survival duration was longer than 30 days. The blood glucose levels in control group were not recovered to normal, and the islet graft survival duration was 4 days. The glucose-lowering rate in experimental group was significantly faster than that in control group and diabetes control group (P < 0.05). Experimental findings indicate that, islets encapsulated in alginate-barium chloride microbeads could survive in the muscles and treat type 1 diabetes mellitus.