不同剂量比淫羊藿苷与辛伐他汀对家兔桡骨骨折愈合的影响

背景：淫羊藿苷与辛伐他汀单独或联合应用均可以促进骨折愈合,联合应用的最佳剂量比缺乏实验依据。目的：观察不同剂量比淫羊藿苷与辛伐他汀对家兔骨折愈合的影响。方法：将45只健康家兔建立双侧桡骨骨折模型,随机等分为模型组、骨形态发生蛋白2组和淫羊藿苷∶辛伐他汀3∶1,1∶1,1∶3组。模型组、骨形态发生蛋白2组给予生理盐水灌胃,淫羊藿苷∶辛伐他汀3∶1,1∶1,1∶3组给予淫羊藿苷溶液15 mL/(kg•d)和辛伐他汀溶液5 mL/(kg•d),淫羊藿苷溶液      10 mL/(kg•d)和辛伐他汀溶液10 mL/(kg•d),淫羊藿苷溶液5 mL/(kg•d)和辛伐他汀溶液15 mL/(kg•d)灌胃。模型组和淫羊藿苷:辛伐他汀3∶1,1∶1,1∶3组骨折局部注射生理盐水,骨形态发生蛋白2组骨折局部注射骨形态发生蛋白2溶液。灌胃连续6周,骨折局部注射连续2周。结果与结论：在骨痂生长、改建和髓腔再通、骨密度和骨折部位组织形态方面,骨形态发生蛋白2、淫羊藿苷:辛伐他汀1∶1组最好,淫羊藿苷:辛伐他汀3∶1,1∶3组居中,模型组最差。提示不同剂量比淫羊藿苷和辛伐他汀联合应用均可促进家兔桡骨骨折愈合,当剂量比为1∶1时,促进作用更显著。

Effect of icariin and simvastatin with different dosage ratios on rabbits’ radial fracture healing

BACKGROUND: Icariin and simvastatin alone or in combination can promote the radial fracture healing of rabbits. But the best dosage ratio is still lack of basis.OBJECTIVE:To investigate the effect of icariin and simvastatin with different dosage ratios on rabbits’ radial fracture healing. METHODS:Forty-five healthy rabbits were enrolled to prepare bilateral radial fracture models, and then randomly divided into five groups: model group; bone morphogenetic protein-2 control group; and three experimental groups (ratio of icariin and simvastatin: 3:1, 1:1, 1:3). Model and bone morphogenetic protein-2 control groups were given normal saline by gastric perfusion. The three experimental groups were given icariin and simvastatin solution with dosage ratios of 3:1, 1:1, 1:3 by gastric perfusion. Model group and three experimental groups were injected normal saline at fracture site, while bone morphogenetic protein-2 control group was injected bone morphogenetic protein-2 solution. Gastric perfusion was performed for 6 consecutive weeks and local injection at fracture site lasted for 2 weeks.RESULTS AND CONCLUSION: Bone morphogenetic protein-2 control group and 1:1 experimental group were the best followed by the 3:1 and 1:3 experimental groups, and the model group was the worst in the following aspects: growth and alteration of callus, recanalization of bone marrow cavity, bone mineral density and morphology of the fracture site. Icariin and simvastatin with different dosage ratios all can promote the radial fracture healing of rabbits. When the dosage ratio is 1:1, the combination of icariin and simvastatin can further promote fracture healing.