Effect of lipid composition on activity of liposome-entrapped ampicillin against intracellular Listeria monocytogenes. |
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Authors: | I A Bakker-Woudenberg A F Lokerse F H Roerdink |
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Affiliation: | Department of Clinical Microbiology and Antimicrobial Therapy, Erasmus University Rotterdam, The Netherlands. |
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Abstract: | The effect of lipid composition on the intracellular antibacterial activity of ampicillin-containing liposomes was studied in vitro by using mouse peritoneal macrophages infected with Listeria monocytogenes. Two types of liposomes, a fluid type, consisting of cholesterol-phosphatidylcholine-phosphatidylserine (5:4:1), and a solid type, consisting of cholesterol-distearoylphosphatidylcholine-dipalmitoylphosphatidylglyc ero l (10:10:1), were used. Although the cellular uptake of both types of liposomes was similar, they differed with respect to the rate of intracellular degradation. A correlation was found between the relatively slow degradation of the solid liposomes and a delayed intracellular release of the encapsulated ampicillin, as reflected in absent or delayed intracellular killing of L. monocytogenes. |
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