凋亡相关基因及蛋白的表达与人卵巢癌细胞顺铂耐药的关系

目的探讨COC1/DDP和COC1中凋亡相关基因及凋亡相关蛋白的表达与人卵巢癌顺铂耐药的关系。方法采用流式细胞仪检测不同浓度顺铂作用于COC1及COC1/DDP细胞48h后细胞凋亡率;RT-PCR及Western blot法检测凋亡相关基因及蛋白Bcl-2、Caspase-3、Smac和Survivin在COC1和COC1/DDP中的表达。结果DDP作用于COC1和COC1/DDP细胞48h后细胞随着DDP浓度的升高,凋亡率也相应增高。COC1/DDP中抗凋亡基因及蛋白Bcl-2、Survivin的表达明显高于COC1。COC1/DDP中促进凋亡的基因及蛋白Smac、Caspase-3的表达明显低于COC1。结论在COC1/DDP中抗凋亡基因及蛋白Bcl-2、Survivin的高表达以及促进凋亡的基因及蛋白Smac和Caspase-3的低表达可能参与COC1/DDP对DDP耐药的发生机制。

Expression of apoptosis-associated proteins and drug resistance in patients with ovarian cancer

Objective] This study was designed to explore the role of apoptosis- associated genes and proteins on the chemo-resistance in COC1/DDP. Methods] The apoptotic ratios of COC1 and COC1/DDP were measured with flow cytometry after treated with different concentration of cisplatin for 48 hours. The expressions of apoptosis- associated genes and proteins Bcl-2, Caspase-3, Smac, Survivin were studied by reverse trans-cription polymerase chain reaction (RT-PCR) and Western blot in COC1/ DDP and COC1. Results] Using DDP to stimulate COC1/DDP and COC1 for 48 hours, the apoptic ratios were gradually increased with the increase of concentration of DDP. The mRNA and protein expressions of Bcl-2 and Survivin in COC1/DDP cells were significantly higher than those in COC1 cells (P <0.05), contrastly Smac and Caspase-3 were significantly lower than those in COC1 cells. Conclusion] The cisplatin-resistance in human ovarian carcinoma cell lines may be associated with the overexpression of anti-apoptic gene and protein of Bcl-2, Survivin and the downregulation of Caspase-3 and Smac.