GM-CSF及B7-1基因修饰的肿瘤细胞疫苗抗肿瘤的研究

目的：研究ＧＭ－ＣＳＦ及Ｂ７－１基因修饰的肿瘤细胞作为瘤苗的有效性。方法：我们将小鼠ＧＭ－ＣＳＦ及ＣＤ８０基因通过逆转录病毒载体分别导入ＥＬ－４淋巴瘤细胞中，进而研究了ＥＬ－４／ＧＭ－ＣＳＦ及ＥＬ－４／Ｂ７－１在同系Ｃ５７Ｂ习中的成瘤性及其诱导抗肿瘤免疫的效果。结果：转Ｂ７－１基因的肿瘤细胞诱发了ＣＤ８０的高表达。转基因的肿瘤细胞在同源小鼠中的成瘤性下降，免疫原笥增强。在肿瘤生长的早期对实验性荷瘤

Anti-Tumor Effect of GM-CSF Gene or B7-1 Gene Modified Murine EL-4 Cell Vaccine

Objective: To investigate the vaccine potency of GM CSF or B7 1 gene modified tumor cells. Methods: The EL 4 lymphoma cells were transduced with recombinant retrovirus containing the murine GM CSF gene or B7 1 gene, respectively. Results: Flow cytometry analysis showed that expression of their surface marker between wild type EL 4 cells and gene transduced tumor cells was the same except for CD80 positive in B7 1 gene transduced cells. GM CSF gene or B7 1 gene transduced EL 4 cells resulted in remarkable loss of tumorigenicity in syngenetic mice. The systemic protective immunity was induced against the challenge with EL 4/wt cells. Therapeutic vaccine with EL 4/GM CSF or EL 4/B7 1 cells could retard the growth of established early stage EL 4/wt tumor significantly, but not retard the growth of late stage EL 4/wt tumor. Irradiated GM CSF gene transduced EL 4 cells showed strong vaccine effect against challenged EL 4 cells, but irradiated B7 1 gene transduced EL 4 cells showed weak vaccine effect. Remarkable cooperative anti tumor effect against challenged EL 4 cells was observed when both irradiated EL 4/GM CSF and EL 4/B7 1 were inoculated together. Conclusion: GM CSF gene or B7 1 gene transduced EL 4 cells can be used as a good tumor vaccine. It suggested that the combination of the two kinds of vaccine has potential application value in human cancer treatment.