PCR法检测乳腺癌患者血清抗p53蛋白抗体的研究

目的为乳腺癌诊断提供血清学新方法.方法用免疫-聚合酶链反应（immuno-polymerase chain reanon,PCR）法检测血清抗p53蛋白（一种抑癌基因）抗体,酶免疫组化方法检测组织p53蛋白表达.结果乳腺癌患者血清抗p53蛋白抗体阳性率为39.5%,而非癌患者和正常人血清抗p53蛋白抗体均为阴性,乳腺癌患者血清中抗p53蛋白抗体显著高于非癌患者和正常人（P＜0.01）.p53蛋白阳性表达的乳腺癌患者抗p53蛋白抗体阳性率为64.2%,明显高于p53蛋白阴性表达组,血清p53抗体测定与p53蛋白表达密切相关（P＜0.01）.结论检测血清抗p53蛋白抗体是检测组织p53蛋白理想的替代工具,抗p53蛋白抗体可以作为乳腺癌血清学诊断新标志,用于乳腺癌的普查和早期诊断.

Detection of seral p53 antibody in patients with breast carcinoma by immuno-polymerase chain reation

Objective To present a new seriological method for diagnosing breast carcinoma.Methods Seral anti-p53 antibody was detected with immuno-polymerase chain reation(Immuno-PCR).The expression of p53 in tissue was detected with enzymeimmu-nohistochemistry technique.Results The positive rate of anti-p53 antibody in serum of breast carcinoma was 39.5% and anti-p53 antibody was not found in non-carcinoma and normal controls.The positive rate of anti-p53 antibody was higher than that of non-carcinoma and normal controls(P<0.01).The positive rate of anti-p53 antibody in positive p53 expression was 64.2% and was higher than that in negative p53 expression.Seral anti-p53 antibody was related to p53 expression in tissue obviously(P<0.01).Conclusion Detecting seral anti-p53 antibody is an alternative tool for detecting p53 expression in tissue.Seral anti-p53 antibody is a new marker for diagnosing breast carcinoma and can be used in early dianosing breast carcinoma.