Effect of Vehicle and Agitation Methods on the Penetration of Calcium Hydroxide Paste in the Dentinal Tubules |
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| Affiliation: | 1. School of Health Sciences, Universidade Positivo, Curitiba, Paraná, Brazil;2. Piracicaba Dental School, Universidade Estadual de Campinas, Piracicaba, São Paulo, Brazil;1. Unidade de Investigação em Ciências Orais e Biomédicas, Faculdade de Medicina Dentária, Universidade de Lisboa, Lisboa, Portugal;2. Centro de Estudo de Medicina Dentária Baseada na Evidência, Faculdade de Medicina Dentária, Universidade de Lisboa, Lisboa, Portugal;3. Instituto de Implantologia, Lisboa, Portugal;4. Department of Endodontics, School of Dentistry, Grande Rio University, Rio de Janeiro, Rio de Janeiro, Brazil;6. Department of Endodontics, Fluminense Federal University, Niteroi, Rio de Janeiro, Brazil;5. LIBPhys-FCT UID/FIS/04559/2013, Lisboa, Portugal;7. Department of Endodontics, Faculdade de Medicina Dentária, Universidade de Lisboa, Lisboa, Portugal;11. CENIMAT/I3N, Department of Materials Science, NOVA School of Science and Technology, Universidade NOVA de Lisboa, Caparica, Portugal;12. Dental Specialty Center, Brazilian Military Police, Minas Gerais, Brazil;1. Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Endodontia, Campinas, São Paulo, Brazil;2. Department of Endodontics, City University of São Paulo, São Paulo, São Paulo, Brazil;3. Department of Endodontics, International University of Catalunya, Barcelona, Spain;1. Konya Research and Training Hospital, Konya, Turkey;2. Research Center of Dental Faculty, Hacettepe University, Ankara, Turkey;3. Department of Periodontology, Selcuk University, Konya, Turkey;4. Department of Endodontics, Selcuk University, Konya, Turkey |
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| Abstract: | IntroductionThe aim of this ex vivo study was to analyze the effect of different vehicles combined with agitation methods on the penetration of calcium hydroxide (CH) paste in the dentinal tubules.MethodsSixty freshly extracted, single-rooted human premolars were prepared using WaveOne Gold (Dentsply Sirona, York, PA) and randomized into 6 experimental groups (n = 10) according to the vehicle and method of paste agitation as follows: propylene glycol (PG) + Lentulo, PG + Lentulo + ultrasound, PG + Lentulo + sonic, distilled water (DW) + Lentulo, DW + Lentulo + ultrasonic, and DW + Lentulo + sonic. The CH paste was manipulated with sodium fluorescein dye and was inserted to completely fill the root canal. Two 1-mm thick sections at 2 mm and 5 mm from the apex of each root were obtained. The sections were scanned using confocal laser scanning microscopy, and the images were analyzed using ImageJ software (Bethesda, MD) to calculate the percentage penetration and maximum penetration depth of CH paste. Statistical analysis was performed using 1-way analysis of variance and Tukey honestly significant difference post hoc tests.ResultsNo statistically significant differences between study factors at a distance of 2 mm from the apex were observed (P > .05). However, at 5 mm, a significant difference between the vehicles (P < .05) in percentage penetration was demonstrated with higher means for PG.ConclusionsVehicles interfered with the penetration of CH in the dentinal tubules at 5 mm from the apex with better results for PG. |
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| Keywords: | Calcium hydroxide confocal microscopy endodontics fluorescent dyes |
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