Self-cured resin modified by quaternary ammonium methacrylates and chlorhexidine: Cytotoxicity,antimicrobial, physical,and mechanical properties |
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Institution: | 1. Department of Prosthodontics, School of Dentistry, Rio de Janeiro State University - UERJ, Rio de Janeiro, RJ, Brazil;2. School of Dentistry, Estácio de Sá University - UNESA, Rio de Janeiro, RJ, Brazil;3. School of Pharmacy, LabTIF, Federal University of Rio de Janeiro – UFRJ, Ilha do Fundão, Rio de Janeiro, RJ, Brazil;4. School of Dentistry, Federal Fluminense University - UFF, Niterói, RJ, Brazil;5. School of Medical Sciences, Rio de Janeiro State University - UERJ, Rio de Janeiro, RJ, Brazil;6. Nucleus for Dental Biomaterials Research, UVA-Veiga de Almeida University, Rio de Janeiro, RJ, Brazil |
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Abstract: | ObjectiveTo evaluate the addition of dimethylaminohexadecyl methacrylate (DMAHDM) and chlorhexidine diacetate on cytotoxicity, antimicrobial activity, physical, and mechanical properties of a self-cured resin.Methods132 disk-shaped and 48 rectangular specimens were divided into four experimental groups as described: Control Group (CG – no addition), dCHX (1%), DMAHDM (5%), and DMAHDM + dCHX (5% + 1%). The biofilm viability, flexural strength (FS - ISO 20795-1:2013), surface roughness (SR), and color stability (ΔE) were analyzed after being stored for 4 weeks in distilled water and immersed for 72 h in coffee. Cytotoxicity was measured after 24 h, 3, and 7 days of elution using an MTT test on L929 cells (ISO 10993-5:2009). SR and ΔE were measured by a contact profilometer and a spectrophotometer using the CIELab parameter. Data were submitted to ANOVA and Bonferroni’s/Tukey’s tests (p ≤ 0.05).ResultsSignificant antimicrobial activity against Streptococcus mutans and Candida albicans was detected in all groups when compared to the CG (p < 0.05). Only the dCHX group, in 24 h of elution, demonstrated no cytotoxicity effects. There was a statistical difference for FS on the tested groups (p < 0.05). No differences were detected in the initial roughness’ measurements among the groups (p > 0.05). However, after storage and immersion in coffee, the groups containing DMAHDM presented with rougher surfaces and significantly lower color stability compared to the control (p < 0.05).SignificanceThe addition of dCHX and DMAHDM in self-cured resin presented antimicrobial properties; however, cytotoxicity, physical, and mechanical properties were compromised. |
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Keywords: | PMMA Chlorhexidine Quaternary of ammonium methacrylate Biofilm inhibition Mechanical properties Physical properties Cytotoxicity |
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