Microtissue Engineering Root Dentin with Photodynamically Cross-linked Nanoparticles Improves Fatigue Resistance of Endodontically Treated Teeth |
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| Institution: | 1. Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada;2. Department of Materials Science and Engineering, University of Toronto, Toronto, Ontario, Canada;1. Department of Restorative Dentistry, Rutgers School of Dental Medicine, Newark, New Jersey;2. Department of Endodontics, University of the Pacific, Arthur A. Dugoni School of Dentistry, San Francisco, California;3. Sector of Angiogenesis Regenerative Medicine, Dr. Hajar Afsar Lajevardi Dental Material and Devices Group, Hackensack, New Jersey;4. Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin;6. Department of Biomedical Engineering, University of Wisconsin, Madison, Wisconsin;1. Department of Restorative Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil;2. OMFS IMPATH Research Group, Department of Imaging and Pathology, Faculty of Medicine, University of Leuven, University Hospitals Leuven, Leuven, Belgium;3. Faculty of Dentistry, University of Ribeirão Preto, Ribeirão Preto, Brazil;4. Department of Dental Medicine, Karolinska Institute, Stockholm, Sweden;1. Department of Endodontics, Faculty of Dentistry, Istanbul Okan University, Istanbul, Turkey;2. Department of DentoMaxillofacial Radiology, Faculty of Dentistry, Istanbul Okan University, Istanbul, Turkey;1. Department of Endodontology, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece;2. Institute of Electronic Structure and Laser, Foundation for Research and Technology–Hellas, Heraklion, Crete, Greece |
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| Abstract: | IntroductionMicrotissue engineering root canal dentin with biopolymeric nanoparticles has the potential to improve mechanical properties of iatrogenically compromised root dentin. This study aims to characterize the surface mechanical property, bulk biomechanical response, and fatigue resistance of microtissue-engineered root dentin using photodynamically (photodynamic-activated PDA]) cross-linked chitosan nanoparticles (CSnps).MethodsExperiments were conducted in 3 parts: part 1, root canal dentin sections were subjected to nanoindentations before/after treatment with CSnps and chemically (1-ethyl-3-3-dimethylaminopropyl]carbodiimide EDC] cross-linked CSnps) and photodynamically cross-linked CSnps to determine the properties of treated surfaces (n = 84 points/group); part 2, root canal dentin specimens treated with PDA cross-linked CSnps were subjected to strain analysis using customized moiré interferometry (n = 5/group); and part 3, root canal dentin specimens treated with EDC cross-linked CSnps, PDA cross-linked CSnps, and instrumented controls were tested using an accelerated fatigue loading protocol to evaluate the sustained loads and cycles at failure (n = 15/group). Data were analyzed using the paired sample t test, trend analysis, and Kaplan-Meier with log-rank tests at a significance of .05 in each experiment.ResultsRoot dentin microtissue engineered with PDA cross-linked CSnps showed a 16.8% increase in elastic modulus and a conspicuous decrease in strain distribution in cervical root dentin (P < .01). There was a significant reduction in the tensile strain formed at the apical region of the instrumented root dentin after treatment (P < .05). Survival analysis showed a statistically significant difference (P < .05) among evaluated conditions in fatigue resistance (ie, PDA cross-linked CSnps > EDC cross-linked CSnps > control).ConclusionsThis study highlighted the potential of root canal dentin microtissue engineering with PDA cross-linked CSnps to diminish radicular strain distribution and improve resistance to fatigue loads in endodontically treated teeth. |
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| Keywords: | Endodontically treated teeth fatigue resistance microtissue-engineered dentin nanoparticles photodynamic cross-link |
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