Mesenchymal Stem Cells Derived from Human Inflamed Dental Pulp Exhibit Impaired Immunomodulatory Capacity In Vitro |
| |
| Affiliation: | 1. Dental School, Universidad de Los Andes, Las Condes, Santiago, Chile;2. Programa de Inmunología Traslacional, Facultad de Medicina, Clínica Alemana Universidad del Desarrollo, Lo Barnechea, Santiago, Chile;1. Unidade de Investigação em Ciências Orais e Biomédicas, Faculdade de Medicina Dentária, Universidade de Lisboa, Lisboa, Portugal;2. Centro de Estudo de Medicina Dentária Baseada na Evidência, Faculdade de Medicina Dentária, Universidade de Lisboa, Lisboa, Portugal;3. Instituto de Implantologia, Lisboa, Portugal;4. Department of Endodontics, School of Dentistry, Grande Rio University, Rio de Janeiro, Rio de Janeiro, Brazil;6. Department of Endodontics, Fluminense Federal University, Niteroi, Rio de Janeiro, Brazil;5. LIBPhys-FCT UID/FIS/04559/2013, Lisboa, Portugal;7. Department of Endodontics, Faculdade de Medicina Dentária, Universidade de Lisboa, Lisboa, Portugal;11. CENIMAT/I3N, Department of Materials Science, NOVA School of Science and Technology, Universidade NOVA de Lisboa, Caparica, Portugal;12. Dental Specialty Center, Brazilian Military Police, Minas Gerais, Brazil;1. Department of Dentistry, State University of Maringá, Maringá, Paraná, Brazil;2. Private Clinic, Belém, Pará, Brazil;3. Private Clinic, Maringá, Paraná, Brazil;4. Statistics Department, State University of Maringá, Maringá, Paraná, Brazil;1. Department of Restorative Dentistry, Rutgers School of Dental Medicine, Newark, New Jersey;2. Department of Endodontics, University of the Pacific, Arthur A. Dugoni School of Dentistry, San Francisco, California;3. Sector of Angiogenesis Regenerative Medicine, Dr. Hajar Afsar Lajevardi Dental Material and Devices Group, Hackensack, New Jersey;4. Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin;6. Department of Biomedical Engineering, University of Wisconsin, Madison, Wisconsin;1. Konya Research and Training Hospital, Konya, Turkey;2. Research Center of Dental Faculty, Hacettepe University, Ankara, Turkey;3. Department of Periodontology, Selcuk University, Konya, Turkey;4. Department of Endodontics, Selcuk University, Konya, Turkey;1. Department of Conservative Dentistry, School of Dentistry, Kyungpook National University, Daegu, Korea;2. Department of Conservative Dentistry, School of Dentistry, Dental Research Institute, Pusan National University, Yangsan, Korea;3. Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, Tennessee;1. Department of Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil;2. Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil;3. Institute of Environmental Sciences, Jagiellonian University, Krakow, Poland;4. Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Zürich, Switzerland |
| |
| Abstract: | IntroductionDental pulp stem cells (DPSC) are very attractive in regenerative medicine. In this study, we focused on the characterization of the functional properties of mesenchymal stem cells derived from DPSCs. Currently, it is unknown whether inflammatory conditions present in an inflamed dental pulp tissue could alter the immunomodulatory properties of DPSCs. This study aimed to evaluate the immunomodulatory capacity in vitro of DPSCs derived from healthy and inflamed dental pulp.MethodsDPSCs from 10 healthy and inflamed dental pulps (irreversible pulpitis) were characterized according to the minimal criteria of the International Society for Cell Therapy, proliferation, differential potential, and colony-forming units. Furthermore, the immunomodulatory capacity of DPSCs was tested on the proliferation of T lymphocytes by flow cytometry and the in vitro enzyme activity of indoleamine 2, 3-dioxygenase.ResultsThere were no significant differences in the DPSC characteristics and properties such as immunophenotype, tridifferentiation, colony-forming units, and proliferation of the DPSCs derived from normal and inflamed pulp tissue. Furthermore, there were significant differences in the immunomodulatory capacity of DPSCs obtained from human healthy dental pulp and with the diagnosis of irreversible pulpitis.ConclusionsOur results showed that DPSCs isolated from inflamed dental pulp showed typical characteristics of MSCs and diminished immunosuppressive capacity in vitro in comparison with MSCs derived from healthy dental pulp. Further investigation in vivo is needed to clarify the mechanism of this diminished immunosuppressive capacity. |
| |
| Keywords: | Healthy dental pulp inflamed dental pulp mesenchymal stem cells pulpitis |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
