Toll-like Receptor Expression Profile of Human Stem/Progenitor Cells Form the Apical Papilla |
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| Affiliation: | 1. Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian-Albrechts University, Kiel, Germany;2. Oral Medicine and Periodontology Department, Faculty of Oral and Dental Medicine, Cairo University, Cairo, Egypt;1. Department of Restorative, Preventive and Pediatric Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland;2. Department of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany;3. WHO Collaborating Centre for Epidemiology and Community Dentistry, University of Milan, Milan, Italy;4. Division of Preventive Dentistry, Periodontology, and Cariology, Department of Endodontology, Center of Dental Medicine, University of Zürich, Zürich, Switzerland;1. Faculty of Dentistry, Department of Oral Biological and Medical Sciences, The University of British Columbia, Vancouver, Bitish Columbia, Canada;2. Department of Conservative Dentistry and Endodontics, Guanghua School of Stomatology, Guangdong Province Key Laboratory of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong Province, China;3. Department of Materials Engineering, The University of British Columbia, Vancouver, Bitish Columbia, Canada;1. Department of Dentistry, Universidade da Região de Joinville, Joinville, Santa Catarina, Brazil;2. School of Health Sciences, Universidade Positivo, Curitiba, Paraná, Brazil;3. Department of Restorative Dentistry, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil;1. Federal University of Paraíba, João Pessoa, Paraíba, Brazil;2. Department of Morphology, Federal University of Paraíba, João Pessoa, Paraíba, Brazil;3. Department of Restorative Dentistry, Federal University of Paraíba, João Pessoa, Paraíba, Brazil;4. Department of Endodontics, State University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil;6. Department of Endodontics, Grande Rio University, Rio de Janeiro, Rio de Janeiro, Brazil;5. Department of Dentistry Clinic, Federal University of Espirito Santo, Vitoria, Espirito Santo, Brazil;1. Department of Endodontics, Ourinhos Integrated School, Ourinhos, São Paulo, Brazil;2. Department of Dentistry, Endodontics, and Dental Materials, School of Dentistry of Bauru, University of São Paulo, São Paulo, São Paulo, Brazil;3. Department of Endodontics, Grande Rio University, Duque de Caxias, Rio de Janeiro, Brazil;1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China;2. Division of Endodontics, Department of Oral Biological & Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, Canada;3. Department of Endodontics, University of Alabama at Birmingham, Birmingham, Alabama |
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| Abstract: | IntroductionStem/progenitor cells from the apical papilla (SCAPs) demonstrate remarkable regenerative and immunomodulatory properties. During their regenerative events, SCAPs, similar to other stem/progenitor cells, could interact with their local inflammatory microenvironment via their expressed toll-like receptors (TLRs). The present study aimed to describe for the first time the unique TLR expression profile of SCAPs.MethodsCells were isolated from the apical papilla of extracted wisdom teeth (n = 8), STRO-1 immunomagnetically sorted, and cultured to obtain single colony-forming units. The expression of CD14, 34, 45, 73, 90, and 105 were characterized on the SCAPs, and their multilineage differentiation potential was examined to prove their multipotent aptitude. After their incubation in basic or inflammatory medium (25 ng/mL interleukin 1 beta, 103 U/mL interferon gamma, 50 ng/mL tumor necrosis factor alpha, and 3 × 103 U/mL interferon alpha), a TLR expression profile for SCAPs under uninflamed as well as inflamed conditions was respectively generated.ResultsSCAPs demonstrated all predefined stem/progenitor cell characteristics. In basic medium, SCAPs expressed TLRs 1–10. The inflammatory microenvironment up-regulated the expression of TLR1, TLR2, TLR4, TLR5, TLR6, and TLR9 and down-regulated the expression of TLR3, TLR7, TLR8, and TLR10 in SCAPs under the inflamed condition.ConclusionsThe present study defines for the first time a distinctive TLR expression profile for SCAPs under uninflamed and inflamed conditions. This profile could greatly impact SCAP responsiveness to their inflammatory microenvironmental agents under regenerative conditions in vivo. |
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| Keywords: | Apical papilla fluorescence-activated cell sorting polymerase chain reaction stem cells toll-like receptor |
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