ECV304细胞用于三维血管新生的研究

目的:采用ECV304细胞(人脐静脉内皮细胞株)构建体外血管新生的三维模型,为ECV304细胞应用于体外血管新生的研究提供依据.方法:制作鼠尾胶原三维凝胶,在凝胶表面种植ECV304细胞,培养至接近汇合状态,换用含碱性成纤维细胞生长因子(bFGF)的培养液继续培养3～12d,用倒置相差显微镜观察内皮细胞株向凝胶内迁移、生长状况.结果:bFGF组ECV304细胞迁移到凝胶内不同层次,形成细胞条索并吻合成网;垂直切面上可见ECV304细胞从凝胶表面的单细胞层向凝胶内发芽生长,形成树枝状毛细血管样结构.结论:ECV304细胞在三维胶原凝胶基质培养模型中,在适当的因子诱导下,能够表现出血管新生中的增殖、迁移、发芽等步骤,可以作为内皮工具来研究体外血管新生.

A three-dimensional model of angiogenesis in vitro

Objective:To develop an experimental three-dimensional model by ECV304 cells(human umbilical vein endothelial cell line) for investigating the mechanisms of angiogenesis in vitro.Methods:ECV304 cells were seeded onto three-dimensional collagen gels made of rat-tail collagen.When the endothelial cells were cultured and grown to near confluence,treated with bFGF for 3 to 12 days,and then assessed with inverted phase contrast microscope.Results:The endothelial cells migrated into the gels,formed complex networks by cell cords at different levels through the bottom view,and sprouted capillary-like structures through the side view.Conclusion:ECV304 cells are capable of expressing some early events of angiogenesis in the three-dimensional collagen gels:proliferating,migrating and sprouting and so on.It should be useful for studying angiogenesis in vitro.