| RP—HPLC法测定铁皮枫斗颗粒中柚皮素含量 |
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| 引用本文： | 颜关秋,陈素红,吕圭源,周桂芬. RP—HPLC法测定铁皮枫斗颗粒中柚皮素含量[J]. 中医药学刊, 2013, 0(9): 1855-1857 |
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| 作者姓名： | 颜关秋 陈素红 吕圭源 周桂芬 |
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| 作者单位： | [1]浙江中医药大学药物研究所,浙江杭州310053 [2]温州医学院中药研究院,浙江温州325035 |
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| 基金项目： | 国家“十二五”科技支撑计划资助项目（2011BA104806）;浙江省卫生高层次创新人才培养工程项目（浙卫发[2010]190号）;浙江省教育厅资助项目（Y201122310）;浙江省重大科技专项项目（2006C13014） |
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| 摘 要： | 目的：建立一种高效液相色谱法测定铁皮枫斗颗粒中柚皮素含量的方法。方法：色谱柱UltimateXB—C18（250mm×4．6mm，5μm）；流动相为0．2％H3PO4的水溶液与甲醇采用梯度洗脱；流速为1．0mL·min^-1；检测波长为280nm；柱温30℃。结果：柚皮素的回归方程为Y=3227．6X-1．7272（r=1．000），线性范围：0．005～0．075μg，加样回收率：100．82％（RSD=1．06％，n=6）。结论：该法准确，阴性制剂无干扰，精密度高，重复性好。可用于铁皮枫斗颗粒中柚皮素的含量测定，并为其它铁皮枫斗相关制剂质量检测提供参考。
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| 关 键 词： | 铁皮枫斗颗粒 铁皮石斛 柚皮素 含量测定 |
Determination of Naringenin in Dendrobium Officinale Granules by HPLC |
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| YAN MeiqiuI,CHEN Suhong,LYU Guiyuanj,ZHOU Guifen. Determination of Naringenin in Dendrobium Officinale Granules by HPLC[J]. Study Journal of Traditional Chinese Medicine, 2013, 0(9): 1855-1857 |
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| Authors: | YAN MeiqiuI CHEN Suhong LYU Guiyuanj ZHOU Guifen |
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| Affiliation: | 1 ( 1. Institute of Materia Medica, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China ; 2. Institute of Chinese Medicine, Wenzhou Medical College,Wenzhou 325035, Zhejiang, China) |
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| Abstract: | Objective：To establish a new HPLC method to determine naringenin in Dendrobium Officinale Granules. Methods：The sample was detected on a ultimate C18 column（250 mm × 4.6 mm,5 μm）with the mobile phase consisting with 0.2% phosphoric acid and methanol by gradient elution at 30 ℃. The flow rate was 1.0 mL · min^-1 ,and wavelength was 280 nm. Results ： The standard curve for naringenin was linear over the range from 0. 005 μg to 0. 075 txg （ r = 1. 000） ,typical equation： Y = 3227.6X - 1. 7272, the average recovery was 100.82% （ RSD = 1.06 % , n = 6）. Conclu- sion：The method shows good accuracy, precision and repeatability. It can be used by quality control of D. Officinale Gran- ules and can be taken as a reference for other connected preparations. |
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| Keywords: | Dendrobium Officinale Granule Dendrobium officinale Kimura et Migo naringenin content determination |
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