丹参酮ⅡA对压力负荷增加大鼠心肌CTGF表达的影响

目的：观察压力负荷增加大鼠心肌组织结缔组织生长因子（ConnectiveTissueGrowthFactor，CTGF）表达的改变，探讨丹参酮ⅡA（TanshinoneⅡA，TanⅡA）对心肌纤维化的保护作用。方法：采用腹主动脉缩窄术制备压力负荷增加心肌纤维化模型，随机分成4组（n=8），假手术组（Sham组）、模型组（Model组）、丹参酮ⅡA组TanⅡ组，20ms／（kg·d）]及阳性对照药物卡托普利组Captopril组，100mg／（kg·d）]。术后4周成功造模并开始给药，疗程为4周。8周后检测心室质量指数、左心室重量指数、心肌组织病理学、心肌羟脯氨酸含量以及心肌组织CTGF蛋白含量。结果：①与Sham组比较，Model组大鼠心脏质量指数和左心脏质量指数明显升高（P〈0．01）；与Model组比较，TanⅡA组则显著降低（P〈0．01）。②与Sham组比较，Model组大鼠心肌羟脯氨酸含量明显升高（P〈0．01）；与Model组比较，TanⅡA组则显著降低（P〈0．01）。⑧与Sham组比较，Model组大鼠心肌组织CTGF表达显著升高（P〈0．01）；与Model组比较，TanIIA组则显著降低（P〈0．01）。结论：TanⅡA组能抑制心肌肥厚，减轻心肌纤维化，此作用可能与下调心肌局部CTGF水平有关。

Expression of Myocardial CTGF in Rats with Pressure Overload and Effect of Tanshinone II A

Objective：To observe the changes of connective tissue growth factor （CTGF） expression in rat myocardial tissue with pressure overload, and to explore the protective effects of Tanshinone II A （Tan II A） on myocardial fibrosis. Methods ： Twenty - four Sprague - Dawley （ SD ） rats induced by the abdominal aorta constriction preparation were random- ly divided into following apporches （n = 8） ： model group （Model group）, Tan II A group Tan I A group 20 mg（/kg · d） ] and positive captopril group captopril group 100 mg/（kg d） ]. The matched age SD sham operation （n =8） was as control. The model of pressure overload - induced myocardial fibrosis was successfully established after 4 weeks of opera- tion. After four weeks of Tan II A treatment, cardiac mass index, left ventricular mass index, histopathological, myocar- dial hydroxyproline content, myocardial CTGF expression were measured. Results ： （!）Compared with sham group, cardiac mass index and left ventricular mass index were increased in Model group （P 〈 0.01 ） ; Compared with Model group, these indexes were decreased in Tan II A group （P 〈 0.01 ）. （2）Compared with sham group, myocardial hydroxyproline content was increased in Model group （P 〈 0.01 ） ; Compared with Model group, Tan II A had significantly reduced myo- Cardial hydroxyproline content （P 〈 0.01 ）. （3）Compared with sham group, CTGF protein content of myocardial tissue was increased in Model group （P 〈 0.01 ） ; Compared with Model group, Tan II A had significantly reduced the expression of CTGF. Conclusions ： Tan II A can inhibit cardiac hypertrophy, attenuate myocardial fibrosis, and this effect may be associ- ated with down-regulation of the expression of fibrogenic cytokines CTGF.