3种植物药单体对肺曲霉生物活性实验

目的：探讨3种植物药单体有效活性成分的抗真菌作用,对肺曲霉进行体外生物活性的MIC和MFC实验。方法：采用试管药基法,将桂皮醛、丁香酚、藿香酮倍比稀释成2.5～0.0098μg/mL的浓度,对致病性肺曲霉（烟曲霉、黄曲霉）进行MIC和MFC实验观察。结果：桂皮醛对烟曲霉MIC均值为0.0391μg/mL,黄曲霉MIC均值为0.0781μg/mL,MFC均为0.0781μg/mL;丁香酚对烟曲霉、黄曲霉MIC均值为0.0781μg/mL,MFC0.1562μg/mL;藿香酮对烟曲霉、黄曲霉MIC均值为0.1562μg/mL,MFC0.3125μg/mL;对照组氟康唑对烟曲霉、黄曲霉MIC和MFC均〉2.5mg/mL。结论：3种植物药单体对烟曲霉、黄曲霉均显示有较强的生物活性,其生物活性依次为桂皮醛、丁香酚、藿香酮。氟康唑对烟曲霉、黄曲霉均显示耐药。

Experimental Study of Bioactivity of Three Kinds of Plant Medicine Monomer aginst Lungs Aspergillus

Objective： The objective of the study was to explore the activity of three monomers of plant medicine against aspergillus and determine the MIC and MFC against pulmonary aspergillus in vitro. Methods： The MIC and MFC values of Cinnamaldehyde, Eugenol and Agastache ketone aginst Aspergillus fumigatus（ A. fumigatus） and aspergillus flavus （ A. flavus） were determined with the drug - medium method. The concentrations of the three drugs were proportionally diluted to 2.5 - 0. 0098 μg/mL. Result ： The Value of MIC and MFC of Cinnamaldehyde against A. fumigatus and A. flavus was 0. 0391 μg/mL, 0. 0781 μg/mL and 0. 0781μg/mL respectively. The value of MIC and MFC of Eugenol a- gainst A. fumigatus and A. flavus was 0. 0781 μg/mL and 0. 1562 μg/mL respectively. The value of MIC and MFC of Agastache ketone aginst A. fumigatus and A. flavus was 0. 1562 μg/mL and 0. 3125 μg/mL respectively. The value of MIC and MFC of fluconazole against A. fumigatus and A. flavus in the control group was more than 2. 5 mg/mL. Conclusions ： The three monomers of plant medicine have strong anti - fungal effects against A. fumigatus and A. flavus, and the effect of Cinnamaldehyde and Eugenol and Agastache ketone increases in order. On the other hand, fluconazote is resistance to A. fumigatus and A. flavus.