寒凝证类痛经大鼠模型建立的探讨

目的：探讨寒凝证类痛经大鼠模型的建立。方法：将处于动情问期的3月龄SD雌性大鼠60只，按照随机数字法分为6组，每组10只，分别为：空白对照组、盐水对照组、类痛经模型组、冷冻5d组、冷冻5d＋给药组、冷冻10d＋给药组。空白大鼠同步抓取不做任何处理，类痛经模型组、冷冻5d组、冷冻5d＋给药组、冷冻10d＋给药组均每日给予大鼠皮下注射苯甲酸雌二醇注射液，连续10d，第1d及第10d日注射0．5mg/只，第2～9d每日0．2nag／只。末次给药1h后，类痛经模型组、冷冻5d＋给药组、冷冻10d＋给药组腹腔注射缩宫素2U／只。盐水组每日给予同等剂量的生理盐水。每天在注射以后，冷冻5d组、冷冻5d＋给药组、冷冻10d＋给药组放入超低温冰柜冷冻4h，在2h时打开冰柜换气5s，冰柜温度维持在～25℃。观察每分钟扭体评分、寒证表征评分、全血还原黏度、红细胞聚集指数以及红细胞刚性指数。结果：与空白对照组比较，冷冻5d组、冷冻5d＋给药组、冷冻10d＋给药组寒证表征评分明显升高（P〈0．01）；类痛经模型组、冷冻5d组和冷冻5d＋给药组的全血还原黏度高切均明显升高（P〈0．05），冷冻5d＋给药组的红细胞刚性指数明显升高（P〈0．05）：与盐水组比较，类痛经模型组、冷冻5d＋给药组、冷冻10d＋给药组的每分钟扭体评分明显升高（P〈0．05）。结论：全身冷冻法可造成大鼠寒凝证；通过寒凝证模型结合类痛经模型的制备方法，成功建立了寒凝证类痛经大鼠模型，该模型符合中医证候模型的造模思路及特点。

Discussion on Establishment of Dysmenorrhea Rat Model with Cold Stagnation Syndrome

Objective ： To explore the establishment of dysmenorrhea rat model with cold stagnation syndrome. Method ： 60 anestrous 3 -month -old female SD rats were divided into six groups according to the random number, n = 10, inclu- ding blank control group, saline control group, dysmenorrhea model group, freeze 5 d group, freeze 5 d ＋ injection group, and freeze 10 d ＋ injection group. Blank rats were synchronically crawled without any treatment. Dysmenorrhea model group, freeze 5 d group , freeze 5 d ＋ injection group, freeze 10 d ＋ injection group were given daily subcutane- ous injection of estradiol benzoate injection for 10 consecutive days, on the first day and lOth day injected 0. 5 mg for each time, during day 2 to day 9, injected 0.2 mg everyday. 1 h after last injection, rats were intraperitoneally injected oxytocin 2 U for dysmenorrhea model group,freeze 5 d ＋ injection group and freeze 10 d ＋ injection group. Saline group were given the same dose of saline. After the injection, the freeze 5 d group, freeze 5 d ＋ injection group and freeze 10 d ＋ injection group were put into the ultra - low temperature freezers for 4 hours, and the freezer ventilation was open at 2 hours for 5 seconds and the freezer was maintained at a temperature of - 25 ℃. The writhing score per minute, cold syndrome characterization score, whole blood reduced viscosity, and erythrocyte aggregation index and erythrocyte rigidity index were observed. Result ： Compared with the blank control group, cold syndrome characterization scores of freeze 5 d group, freeze 5 d ＋ injection group and freeze 10 d ＋ injection group were significantly higher （P 〈 0.01 ） ; full blood re- duced viscosity high shear of dysmenorrhea model group, freeze 5d group and freeze 5 d ＋ injection group were signifi- cantly increased （P 〈 0.05）, erythrocyte rigidity index of freeze 5 d ＋ injection group was significantly higher （ P 〈 0.05） ; compared with the saline group, writhing score per minute of dysmenorrhea model group, freeze 5 d ＋ injection group and freezel0 d ＋ injection group was significantly higher （P 〈 0.05 ）. Conclusion： Systemic freezing can cause cold stagnation syndrome in rats. To combine preparation of dysmenorrhea model with cold stagnation model,we success- fully established the dysmenorrhea rat model with cold stagnation syndrome.