| 大鼠血浆中棉酚的检测简 |
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| 引用本文： | 栾姝,姚军,林晓密.大鼠血浆中棉酚的检测简[J].天津药学,2013(5):1-3. |
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| 作者姓名： | 栾姝 姚军 林晓密 |
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| 作者单位： | [1]辽东学院医学院,丹东118003 [2]新疆医科大学药学院,乌鲁木齐830054 |
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| 基金项目： | 新疆维吾尔自治区高校科研计划青年教师科研启动基金项目（No.XJYDU2007S21） |
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| 摘 要： | 目的：建立大鼠血浆中棉酚含量的高效液相色谱测定方法。方法：采用ODS—C18色谱柱（4．6mm×200mm，5μm），柱温25℃，流动相为甲醇-1％磷酸溶液（85：15），流速1．0ml／min，检测波长：235nm。结果：在0．33～3．30μg/ml范围内峰面积与浓度呈现良好的线性关系（r=0．9994），检测限为0．04μg/ml；平均回收率为94．7％～97．3％；给药组大鼠血浆中棉酚的含量为0．68～2．14μg／ml。结论：该方法可用于棉酚生物样品分析。
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| 关 键 词： | 高效液相色谱法 大鼠 棉酚 |
Determination of gossypol in rat plasma by HPLC |
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| Luan Shu,Yaojun,Lin Xiaomi.Determination of gossypol in rat plasma by HPLC[J].Tianjin Pharmacy,2013(5):1-3. |
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| Authors: | Luan Shu Yaojun Lin Xiaomi |
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| Institution: | 1. College of Medical, Eastern Liaoning University, Dandong 118003 ; 2. College of Pharmacy, Xinjiang Medical University, Urumqi 830054 ) |
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| Abstract: | Objective ：To establish an HPLC method for determination of gossypol in rat plasma. Methods ：An ODS -C18 column （4.6 mm ×200 mm, 5 μm） was used as stationary phase, and methanol and 1% phosphoric acid solution （85： 15, v/v） as mobile phase with the flow rate of 1.0 ml/min. The detective wavelength was at 235 nm. Results ：The peak area of gossypol and its concentration showed a good linear relationship when the concentrations varied from 0.33 - 3.30 μg/ml, related coefficient being 0. 999 4. The limit of detection was 0.04μg/ml （ S/N = 3 ）. The relative recovery was between 94.7% and 97.3%. The content of gossypol in rat plasma was between 0.68 μg/ml and 2.14μg/ml. Conclusion：This method can be used for analysis of gossypol in biological samples. |
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| Keywords: | HPLC rat plasma gossypol |
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