狼毒乙素和新狼毒素A的抗肿瘤作用

目的：观察狼毒乙素（ECB）对 U14宫颈癌的荷瘤小鼠，以及新狼毒素A对雄激素非依赖性前列腺癌（AIPCa）裸鼠移植瘤的抑制作用。方法：① U14宫颈癌的腋下移植瘤小鼠各50只，各随机分成5组，实验组灌胃分别给予狼毒乙素（ECB）高、中、低组（12、10、8mg／kg），阳性药组灌胃给予环磷酰胺（50mg／kg），模型组给予等量生理盐水，给药14d后处死动物剥离瘤组织称质量，计算抑瘤率。②雄性裸小鼠20只，皮下接种DU-145细胞，随机分成用药组和对照组。于癌细胞种植第2天起，对照组每天喂饲0．9％氯化钠溶液，用药组每天喂饲新狼毒素A15rag·kg-1·d-1。记录用药第2—7周末时移植瘤的平均体积。结果：①狼毒乙素（ECB）高、中、低剂量组对小鼠 U14宫颈癌的抑瘤率分别为66，40％，49，61％，44．14％；②用药组裸小鼠移植瘤的生长明显受到抑制，第3周末至第7周末的移植瘤体积均显著小于对照组（P值均〈0．001）。第7周结束时与对照组比较，用药组的生长抑制率达到65．34％。结论：狼毒乙素（ECB）对小鼠宫颈癌U14有显著抑制作用；新狼毒素A可以显著抑制DU-145肿瘤的生长，提示该药对AIPCa有抗癌活性。

Anti-tumor Effects of ECB and NCA

Objective ：To research the inhibition effect of ECB on U14 cervical cancer and NCA on androgen independ- ent prostate cancer（AIPCa） xenografts in nude mice. Methods：l. Fifty cervical cancer bearing mice were randomly divid- ed into five groups, which received ECB high - dose, middle - dose and low - dose （ 12 mg/kg, 10 mg/kg, 8 mg/kg） , cyclophosphamide （50 mg/kg） and saline respectively for 14 days. The tumor tissues were cut off,weighed and the inhi- bition rate was calculated. 2. DU - 145 cells were subcutaneously injected to 20 male BALB/c - nude mice and those mice were randomly divided into administration group and control group. From day 2 after the injection, the mice were fed with 0. 9% saline solution in the control group and NCA（ 15 mg. kg- 1 .＇ d- 1 ）in the NCA group every day. The aver- age size of xenografts was measured every week from week 2to week 7. At the end of week 7, all mice were executed and the tumors were removed. Results：l. The inhibition rate of U14 cervical cancer was 66.40% for high - dose ECB（ P 〈 0. 01 ） ,49.61% for middle - dose ECB （ P 〈 0.01 ） , 44.14 % for secondary low ECB H Dripping Pills （ P 〈 0.01 ） . 2. The growth of xenografts in the NCA group was significantly inhibited, and the tumors were significantly smaller than those in the control group from the end of week 3 to week 7 （ all P 〈 0. 001 ）. Compared with control group, the growth inhibiting rate of the tumors in the NCA group was 65.34% at the end of week 7. Conclusion.ECB can significantly in- hibit the growth of U14 cervical cancer bearing mouse;NCA can obviously inhibit the growth of DU- 145 cells,indicating that NCA has the anti -tumor activity to AIPCa.