复合造模法建立大鼠胃癌前病变模型的实验研究

目的：建立大鼠胃癌前病变的动物模型，缩短造模时间，提高诱变率。方法：将雄性Wistar大鼠80只随机分成4组，即空白组，A组单纯MNNG造模，B组复合无酒精造模和c组复合加酒精造模。比较大鼠各组造模时间、病理变化情况及诱变率。结果：①C组造模时间为12周，造模时间最短。诱变率为72．23％，明显优于其他两组（P〈0．01）。②酒精在40℃下，能提高MNNG溶解性和诱变率。结论：①复合造模法成功建立大鼠胃癌前病变的动物模型。②酒精可以促进MNNG溶解，提高诱发率，缩短造模时间。

Experiment Study on Composite Method in Establishment of Gastric Precancerous Lesions Change in Rats Model

Objective ： To establish the model of gastric precancerous lesions in rats to shorten the time of PLGC mod- el. Methods ： Eighty male Wistar rats were divided into 4 groups randomly ： control group, MNNG group （ group A）, com- posite factors without alcohol group（ group B） ;composite factors with alcohol group（ group C）. The model time, patholog- ical changes and rate of mutagenic can be compared with each group in rats. Results： ①The model time of group C was 12 weeks, which was the shortest time. The rate of mutagenic was 72.23% and was much better than that of the other two groups （P 〈0.01 ）. ②The alcohol in 40% can enhance the MNNG solubility and the successful rate. Conclusion： ③Composite method established gastric precancerous lesions in rats successfully. ④The alcohol enhanced the MNNG sol- ubility, shortened the time of model.