EPO促进血管痴呆大鼠神经干细胞增殖的机制研究

目的研究促红细胞生成素（EPO）促进血管性痴呆（VD）大鼠神经干细胞（NSC）增殖的分子调控机制。方法选取SD大鼠60只,采用改良血管阻断加硝普钠降压法建立VD大鼠模型,随机分为VD组和治疗组;治疗组腹腔注射EPO,分别于建模后7、14 d提取分离各组大鼠脑组织RNA,采用RT-PCR检测NSC增殖相关调控基因FGF2、EGF、TGFа表达。结果治疗组7 d、14 d时的FGF2、EGF表达均高于VD组（P〈0.05）,TGFа表达两组无统计学差异;7 d时两组FGF2表达明显高于EGF（P〈0.05）,14 d时则无统计学差异。结论 EPO通过上调胞外FGF2及EGF表达促进VD大鼠NSC增殖;在VD大鼠NSC增殖早期,FGF2起主要作用。

Mechanism of erythropoietin in promoting the proliferation of neural stem cells in rats with vascular dementia

Objective To explore the molecular mechanism of erythropoietin（EPO）in promoting the proliferation of neural stem cells（NSC） in rats with vascular dementia（VD）.Methods 60 SD rats were selected and the modified vessel occlusion and depressurization with sodium nitroprusside were applied to the establishment of VD rat models;the 60 VD rats were randomly divided into2 groups：VD group and treatment group;intraperitoneal injection of EPO was made to rats in treatment group,while the same amount of saline to rats in VD group;RNA isolated from rat brain tissue was extracted at the time point of 7 days and 14 days after the model establishment;RT-PCR was applied to detect the expression of FGF2,EGF and TGFa,the regulatory genes related to NSC proliferation.Results The expressions of FGF2 and EGF in treatment group at the time point of 7 days and 14 dyas after the model establishment were higher than those in VD group（P〈0.05）,while there existed no statistical difference in the expression of TGFa between the 2 groups;the expression of FGF2 was higher than that of EGF at the time point of 7 days after the model establishment in both groups,while there existed no statistical difference between the expression of FGF2 and that of EGF at the time point of 14 days after the model establishment in both groups（P〈0.05）.Conclusions EPO promotes the proliferation of NSC in VD rats by up-regulating the expressions of extracellular FGF2 and EGF;FGF2 plays a main role in the early proliferation of NSC in VD rats.