老年肺结核患者外周血NKG2A、NKG2D表达及相关分析

目的了解老年肺结核患者NKG2A、NKG2D表达水平的变化情况,为肺结核免疫治疗提供新思路和理论依据。方法应用流式细胞术分析100例老年初治肺结核患者和89例老年健康体检者外周血中NK细胞表面受体NKG2A、NKG2D表达水平。结果结核组与体检组相比,NKG2D表达明显降低,NKG2A表达明显上调;单纯肺结核患者与肺结核合并肺外结核患者相比,后者NKG2A表达更高（P〈0.05）;在结核组中,NKG2D表达率随着病灶受累范围增加而明显下降（P〈0.05）;在菌阴肺结核组与菌阳肺结核组、空洞组与无空洞组之间,NKG2A、NKG2D表达率的差异均无统计学意义（P〉0.05）。结论测定老年初治肺结核患者外周血NKG2A、NKG2D,对于了解其机体免疫功能状态、估计病情具有一定临床价值,为免疫治疗提供重要理论依据。

Expression and analysis of NKG2A and NKG2D in peripheral blood of senile patients with pulmonary tuberculosis

Objective To know the change of expression level of NKG2A and NKG2D of senile patients with pulmonary tuberculosis in order to provide new ideas and theoretical basis for immune therapy of pulmonary tuberculosis. Methods Flow cytometry （FCM） was used to analyze the expression level of NK cells surface receptors NKG2A and NKG2D in peripheral blood of 100 senile patients with early fpulmonary tuberculosis and 89 senile healthy people, respectively. Results Compared with the control group,the expression level of NKG2D in the tuberculosis group decreased significantly while that of NKG2A increased significantly; compared with the patients with pulmonary tuberculosis complicated with extra pulmonary tuberculosis, the patients with simple pulmonary tuberculosis had lower expression level of NKG2A （P 〈 0.05 ） ;in the tuberculosis group, the expression rate of NKG2D decreased significantly with the lesion involvement range increase （ P 〈 0.05 ） ; between the smear-negative pulmonary tuberculosis group and smear-positive pulmonary tuberculosis group and between the pulmonary cavity group and non-cavity group, the expression levels of NKG2A and NKG2D had no statistical difference （ P 〉 0.05 ）. Conclusion The determination of the expression level of NKG2A and NKG2D in peripheral blood of the senile patients with pulmonary tuberculosis has a certain clinical value for understanding the immune function of the patients, and it can provide important theoretical basis for immunotherapy.