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| 结肠黑变病相关基因的cDNA芯片筛查分析 | |
| 引用本文： | 刘俊,田德安,王俊平,郝玉霞,冯璟,赵志忠,汪蓉,张素珍.结肠黑变病相关基因的cDNA芯片筛查分析[J].山西医科大学学报,2008,39(11). |
| 作者姓名： | 刘俊 田德安 王俊平 郝玉霞 冯璟 赵志忠 汪蓉 张素珍 |
| 作者单位： | 1. 华中科技大学同济医学院附属协和医院消化科,武汉,430022;山西省人民医院消化科 2. 华中科技大学同济医学院附属协和医院消化科,武汉,430022 3. 山西省人民医院消化科 4. 山西省肿瘤医院消化科 |
| 基金项目： | 山西省卫生厅科技攻关项目 |
| 摘 要： | 目的 应用基因芯片技术研究结肠黑变病(MC)和正常成人结肠组织基因的差异表达. 方法 分别用Cy5和Cy3两种不同的荧光染料通过逆转录反应将MC组和对照组结肠组织的mRNA分别标记成探针,并与载有一组靶基因的基因表达谱芯片进行杂交.通过扫描荧光强度,计算机软件分析,寻找两组差异表达基因. 结果 MC组和正常对照组之间共筛选出93条差异表达基因,其中表达增加的基因有53条(2.0倍以上),表达降低的基因有40条(0.5倍以下). 结论 基因表达谱芯片筛选MC与正常成人结肠组织差异表达基因具有样品用量少、高速度、高敏感、高通量等特性.通过筛选所得差异基因提示MC的发病可能涉及细胞增殖、酯类代谢、能量代谢、细胞毒等多种因素,为进一步阐明MC的发病机制提供了新线索. |
| 关 键 词： | 结肠黑变病 基因芯片 差异表达 |
Study on differentially expressed genes in melanosis coli by cDNA microarray |
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| LIU Jun,TIAN De-an,WANG Jun-ping,HAO Yu-xia,FENG Jing,ZHAO Zhi-zhong,WANG Rong,ZHANG Su-zhen.Study on differentially expressed genes in melanosis coli by cDNA microarray[J].Journal of Shanxi Medical University,2008,39(11). | |
| Authors: | LIU Jun TIAN De-an WANG Jun-ping HAO Yu-xia FENG Jing ZHAO Zhi-zhong WANG Rong ZHANG Su-zhen |
| Institution: | 1Division of Gastroenterology;Union Hospital of Tongji Medical College;Huazhong University of Science and Technology;Wuhan 430022;China;2Division of Gastroenterology;Shanxi Provincial People's Hospital;3Division of Gastroenterology;Shanxi Provincial Tumor Hospital |
| Abstract: | Objective To explore the differences of the differentially expressed genes in colon tissues of patients with Melanosis coli(MC)and normal controls by cDNA microarray. Methods The mRNA from MC and normal colon tissues were reversely transcribed to cDNA with incorporation of fluorescent dCTP(Cy5 or Cy3)to prepare for hybridization probes.The mixed probes were hybridized with the cDNA microarray.The differential genes in 2 groups were detected by scanning the fluorescent signals. Results All together,93 differ... |
| Keywords: | melanosis coli cDNA microarray differential expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |