六味地黄丸对D-半乳糖所致衰老大鼠学习记忆的改善作用及机理

目的:研究六味地黄丸对D-半乳糖(D-gal)所致衰老大鼠学习记忆能力的影响及作用机制。方法:以D-gal 75 mg.kg-1.d-1皮下注射(s.c)给药8周复制亚急性衰老动物模型,分别灌胃不同剂量的六味地黄丸进行治疗,治疗8周后,用Morris水迷宫检测大鼠的学习记忆能力,测定脑组织单胺氧化酶(MAO)、乙酰胆碱脂酶(AchE)活性。结果:高、低剂量的六味地黄丸可使大鼠空间搜索和辨别的学习能力增强,使脑组织MAO、AchE降低。结论:六味地黄丸可以改善D-gal致衰老大鼠学习记忆障碍。协调中枢胆碱能系统和肾上腺素能系统的功能是其可能的作用机制。

Cognition Enhancing Effect of Liuwei Dihuang Pill on Deterioration of Learning and Memory induced by D-Galactose in Rats

Objective:To probe the cognition enhancing effect of Liuwei Dihuang pill on deterioration of learning and memory induced by D-galactose(D-gal)in rats,and to study the mechanism.Methods:Sub-acute aged animal model was replicated by administration of 75 mg·kg^-1·d^-1 s.c.for 8 weeks.Meanwhile,treating with different dose of Liuwei Dihuang pill for 8 weeks.The learning and memory ability was observed by Morris water maze.Activities of mono amine oxidase(MAO) and acety lcholinesterase(AchE) in brain tissue were detected.Results:High and low dose Liuwei Dihuang pill can enhance learning and memory ability.It can also decrease activities of mono amine oxidase(MAO) and acetylcholinesterase(AchE) in brain tissue.Conclusions:Liuwei Dihuang pill can improve the deterioration of learning and memory induced by D-gal in rats.The possible mechanism are regulating of central cholinergic nerve system and noradrenergic nerve system.