Fe~(2+)对人脐静脉平滑肌细胞增殖及分泌基质金属蛋白酶-2的影响

目的探讨Fe2+对高糖预孵育的人脐静脉平滑肌细胞(human umbilical vascular smooth muscle cell,HUSMC)增殖及基质金属蛋白酶-2(MMP-2)分泌的影响。方法取高糖DMEM培养液培养的原代HUSMC(3~5代),分为空白对照组(加DMEM培养液)、A组(100 mg/L Fe2+培养液)、B组(50 mg/L Fe2+培养液)、C组(25 mg/L Fe2+培养液)、D组(12.5 mg/L Fe2+培养液)、E组(6.25 mg/L Fe2+培养液)。干预24 h后,MTT比色法测定细胞增殖,ELISA检测MMP-2含量,激光共聚焦显微镜测定细胞内Ca2+浓度。结果除E组外其他各Fe2+组促血管平滑肌细胞(VSMC)增殖作用均高于空白对照组(P<0.01),A组、B组作用最强;各浓度Fe2+组MMP-2含量均高于空白对照组(P<0.05);A组细胞内Ca2+荧光强度较空白对照组强(P<0.01)。结论Fe2+能明显促进VSMC增殖,并促进分泌MMP-2,升高细胞内Ca2+浓度。

Effects of Fe2+ on proliferation and secretion of MMP-2 in umbilical venous smooth muscle cells

Objective To investigate the effects of Fe2+on proliferation and secretion of MMP-2 in HUVSMC incubated in high glucose DMEM.Methods Primary cultures of HUVSMCs incubated in the high glucose DMEM(Passage 3～5),were divided into control group and groups in cubated with Fe2+ at five different concentrations(100 mg/L,50 mg/L,25 mg/L,12.5 mg/L,6.25 mg/L).After 24 h of intervention,HUVSMCs proliferation was assessed with MTT method,MMP-2 secretion was measured with ELISA,and the intracellular Ca2+ concentration was assessed by laser confocal microscopy.Results Compared with the control group,HUVSMCs proliferation in 12.5～100 mg/L Fe2+ treatment group was increased(P<0.05);the secretion of MMP-2 was promoted in all Fe2+concentration groups(P<0.05);and the intracellular Ca2+concentration of HUVSMC was augmented by 100 mg/L Fe2+ treatment(P<0.01).Conclusions Fe2+can stimulate the proliferation of VSMC,and increase the intracellular Ca2+ as well as the secretion of MMP-2.