移植NT-4基因修饰细胞对大鼠前角运动神经元损伤的保护作用

目的：观察神经营养素４（ＮＴ－４）基因修饰细胞对成年大鼠脊髓前角运动神经元损伤的保护作用。方法：应用逆转录病毒介导的体外ＮＴ－４基因转移方法，制备高表达ＮＴ－４的基因修饰细胞。离断大鼠左侧坐骨神经作为外周神经损伤模型，右侧作为对照，在离断外移植ＮＴ－４基因修饰细胞。观察大鼠脊髓前角运动神经元尼氏染色和胆碱酯酶染色的变化。结果：在移植ＮＴ－４基因修饰细胞组和对照组间，尼氏和胆碱酯酶染色有显著性差异。

The protective effect of grafting NT-4 genetically modified cells on injury of spine motoneurons in rats

Objective: To study the protective effect of grafting NT 4 genetically modified cells on injury of spine motoneurons in rats. Methods: NT 4 genetically modified cells were obtained by infecting L 6TG cell in vitro with a retroviral vector pN 2A containing the rat NT 4 cDNA gene. The rats with left sciatic nerve apocoped were taken as model for treatment by implanting NT 4 genetically modified cells. The right sciatic nerve was taken as control. The Nissl staining and ChE staining in motoneurons of spine were also studied. Results: There were significant differences between groups of NT 4 and control in the Nissl staining and ChE staining. Conclusion: Grafting NT 4 cells can prevent most motoneurons from death.