染料木黄酮对去势大鼠骨代谢的影响

目的:研究染料木黄酮(Gen)对去势大鼠骨代谢的影响。方法:雌性Wistar大鼠47只,随机分为假手术组,去势对照组、去势+雌激素组[己烯雌酚20μg/(kgbw?d)]、去势+Gen组[剂量分别为25、50、100mg/(kgbw?d)]。饲养3个月后处死,测定骨密度(BMD)、股骨形态学参数和血清骨钙素(BGP)及尿中羟脯氨酸(HYPRO)和吡啶酚(PYR)含量。结果:大鼠去势后,股骨BMD降低,骨小梁体积减少、密度降低、间隙增加。与假手术组比均有显著差异,血清BGP与尿中PYR排出量明显高于假手术组,尿中HYPRO含量也有所增加;补充Gen后,股骨BMD有改善的趋势,骨小梁体积增加、厚度和密度增大,BGP含量降低、尿中HYPRO有降低的趋势,而尿中PYR含量明显降低。结论:染料木黄酮可通过抑制骨转换和破骨细胞活性,减少去势大鼠骨量丢失,预防骨质疏松的发生。

EFFECT OF GENISTEIN ON BONE METABOLISM IN OVAR IECTOMIZED RATS

Objective: To investigate the effects of genistein (Gen) on bone turnover in ovariectomized rats. Methods: Forty-seven Wistar rats were randomly allocated into six groups: sham-operated (sham), ovarietomized (ovx), ovarietomized plus diethyl stilbestrol [E, 20μg/(kg bw·d)] or Gen [25、50、100 mg/(kg bw·d)] administration. After the rats had been fed for 3 mo,bone mineral density (BMD), histomorphometric parameters, the serum concentration of osteocalcin (BGP) and urinary contents of pyridine and hydroxyproline (HYPRO) were analysed. Results: BMD, trabecular bone volume (TBV) and mean trabecular plate density (MTPD) of ovariectomized rats were significantly decreased, but serum concentrations of BGP,urinary contents of deoxypyridinoline and HYPRO and mean trabecular plate space (MTPS) increased compared with the sham. After 3 mo of Gen supplementation, TBV,MTPD and urinary deoxypyridinoline were improved significantly in ovariectomized rats. Conclusion: Genistein reduced bone loss in ovariecromized rats by suppressing bone metabolism and osteoclast activity.