Stimulatory effects of bestatin on human B-cell colony formation |
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| Affiliation: | 1. Department of Anesthesiology and Pain Medicine, Seoul National University Hospital, Seoul, Republic of Korea;2. Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea;1. Department of Clinical Laboratory, Peking University People''s Hospital, Beijing, China;2. Department of Radiology, The First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, Xinjiang, China;1. Division of Cardiology, Department of Pediatrics, The Labatt Family Heart Centre, The Hospital for Sick Children, University of Toronto, Toronto, Canada;2. Division of Pediatrics, P. Universidad Catolica de Chile, Santiago, Chile;3. Division of Pediatric Cardiology, Perth Children´s Hospital, Perth, Australia |
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| Abstract: | Bestatin, (2S, 3R)-3-amino-2-hydroxy-4-phenylbutyryl-L-leucine, is a small molecular immunomodifier. Effects of this compound on human immune function were studied, in vitro, using the human B-cell colony formation technique. B-cell colonies were obtained from enriched B-cell populations placed in conditioned methylcellulose medium containing stimulators and irradiated T-cells as feeders. Addition to the culture of Bestatin at concentrations of 0.1 μg/ml and 1 μg/ml led to a significant increase (P < 0.05) in the number of B-cell colonies and this effect was abolished when irradiated T-cells were not added to the culture. Bestatin increased soluble factor production induced by phytohemagglutinin (PHA)-stimulated T-cells. Such findings suggest that T-cells probably mediate this stimulatory effect of Bestatin on B-cell colony formation. |
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