Simplified Synthesis and Stability Assessment of Aflatoxin B1-Lysine and Aflatoxin G1-Lysine |
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| Authors: | Justin B. Renaud Jacob P. Walsh Mark W. Sumarah |
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| Affiliation: | 1.London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON N5V 4T3, Canada;2.Department of Chemistry, University of Western Ontario, London, ON N6A 3K7, Canada |
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| Abstract: | Aflatoxins B1 (AFB1) and G1 (AFG1) are carcinogenic mycotoxins that contaminate crops such as maize and groundnuts worldwide. The broadly accepted method to assess chronic human aflatoxin exposure is by quantifying the amount of aflatoxin adducted to human serum albumin. This has been reported using ELISA, HPLC, or LC-MS/MS to measure the amount of AFB1-lysine released after proteolysis of serum albumin. LC-MS/MS is the most accurate method but requires both isotopically labelled and unlabelled AFB1-lysine standards, which are not commercially available. In this work, we report a simplified synthetic route to produce unlabelled, deuterated and 13C6 15N2 labelled aflatoxin B1-lysine and for the first-time aflatoxin G1-lysine. Additionally, we report on the stability of these compounds during storage. This simplified synthetic approach will make the production of these important standards more feasible for laboratories performing aflatoxin exposure studies. |
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| Keywords: | aflatoxin biomarker aflatoxin B1-lysine aflatoxin G1-lysine |
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