不同炮制方法对巴戟天中寡糖类成分和水晶兰苷含量的影响

目的:考察不同炮制工艺对巴戟天中寡糖类成分和水晶兰苷含量的影响。方法:采用不同方法制备巴戟天炮制品,比较不同炮制品中寡糖类成分和水晶兰苷的含量。利用HPLC-CAD测定寡糖类成分含量,Shodex Asahipak NH2色谱柱,流动相乙腈(A)-水(B)梯度洗脱(0~15 min,86%~81%A;15~20 min,81%~75%A;20~35 min,75%~70%A;35~50 min,70%~58%A;50~60 min,58%~40%A;60~65 min,40%~20%A);运用HPLC-DAD测定水晶兰苷含量,Venusil MP C18色谱柱,检测波长235 nm,流动相甲醇-0.4%磷酸溶液梯度洗脱(5∶95~28.8∶71.2,15 min)。结果:巴戟天不同炮制品中寡糖类成分总质量分数顺序为盐巴戟天>巴戟肉>制巴戟天>生巴戟天;水晶兰苷含量排序为盐巴戟天>生巴戟天>制巴戟天>巴戟肉。结论:巴戟天不同炮制品中寡糖类成分和水晶兰苷的含量差异较大,为巴戟天的饮片质量控制及炮制机制解析提供参考。

Effects of Different Processing Methods on Concents of Oligosaccharides and Monotropein in Morindae Officinalis Radix

Objective: To investigate effects of different processing methods on concents of oligosaccharides and monotropein in Morindae Officinalis Radix. Method: Processed products of Morindae Officinalis Radix were prepared by different methods,contents of oligosaccharides and monotropein in different processed products were compared.The content of oligosaccharides was determined by HPLC-CAD with Shodex Asahipak NH₂ column,mobile phase of acetonitrile(A)-water(B) for gradient elution (0-15 min,86%-81%A;15-20 min,81%-75%A;20-35 min,75%-70%A;35-50 min,70%-58%A;50-60 min,58%-40%A;60-65 min,40%-20%A);the content of monotropein was determined by HPLC-DAD with Venusil MP C₁₈ column,mobile phase of methanol-0.4% phosphoric acid for gradient elution (5:95-28.8:71.2,15 min) and detection wavelength at 235 nm. Result: The content of oligosaccharides was in the order of Morindae Officinalis Radix steamed by salt >Morindae Officinalis Radix without the wood >Morindae Officinalis Radix processed with Glycyrrhizae Radix et Rhizoma >crude Morindae Officinalis Radix;while according to the content of monotropein was Morindae Officinalis Radix steamed by salt >crude Morindae Officinalis Radix >Morindae Officinalis Radix processed with Glycyrrhizae Radix et Rhizoma >Morindae Officinalis Radix without the wood. Conclusion: Processing could greatly change contents of oligosaccharides and monotropein in Morindae Officinalis Radix,which provided a reference for pieces quality control and processing mechanisms analysis of Morindae Officinalis Radix.