| 反相高效液相色谱法同时测定天麻中天麻素和3种核苷 |
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| 引用本文： | 刘红,陈燕芹,罗树常,夏志,蔡丽.反相高效液相色谱法同时测定天麻中天麻素和3种核苷[J].中国实验方剂学杂志,2014,20(17):81-83. |
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| 作者姓名： | 刘红 陈燕芹 罗树常 夏志 蔡丽 |
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| 作者单位： | 1. 毕节学院化学化工实验教学中心,贵州毕节,551700
2. 贵州省应用化学特色重点实验室,贵州毕节,551700 |
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| 基金项目： | 贵州省科技厅联合基金项目（黔科合J字LKB[2013]05号）；贵州省教育厅大学生创新项目（201310668005）；毕节学院实验室开放基金项目（毕院教OLFNO[2013]120020号） |
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| 摘 要： | 目的:建立同时测定天麻中天麻素和3种核苷含量的RP-HPLC方法。方法:采用ZORBAX-SB-C18色谱柱(4.6mm×150 mm,5μm);流动相0.1%磷酸水溶液-甲醇梯度洗脱,柱温25℃,流速0.6 mL·min-1,检测波长270 nm。结果:天麻素、尿苷、鸟苷和腺苷分别在0.286~4.29μg(r=0.999 9),0.028 9~0.433 5μg(r=0.999 7),0.066 8~1.002μg(r=1.000 0),0.135 6~2.034μg(r=1.000 0)与峰面积线性关系良好。结论:该测定方法简便、快速、准确,可以作为天麻的质量控制方法之一。
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| 关 键 词： | 天麻素 尿苷 鸟苷 腺苷 高效液相色谱 |
| 收稿时间： | 2014/1/20 0:00:00 |
Determination of Three Glycosides and Gastrodine Contents in Gastrodiae Rhizoma by RP-HPLC |
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| LIU Hong,CHEN Yan-qin,LUO Shu-chang,XIA Zhi and CAI Li.Determination of Three Glycosides and Gastrodine Contents in Gastrodiae Rhizoma by RP-HPLC[J].China Journal of Experimental Traditional Medical Formulae,2014,20(17):81-83. |
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| Authors: | LIU Hong CHEN Yan-qin LUO Shu-chang XIA Zhi and CAI Li |
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| Institution: | Experiment Teaching Center for Chemistry and Chemical Engineering, Bijie University, Bijie 551700, China;Key Laboratory on Applied Chemistry, Bijie 551700, China;Experiment Teaching Center for Chemistry and Chemical Engineering, Bijie University, Bijie 551700, China;Key Laboratory on Applied Chemistry, Bijie 551700, China;Experiment Teaching Center for Chemistry and Chemical Engineering, Bijie University, Bijie 551700, China;Key Laboratory on Applied Chemistry, Bijie 551700, China;Experiment Teaching Center for Chemistry and Chemical Engineering, Bijie University, Bijie 551700, China;Key Laboratory on Applied Chemistry, Bijie 551700, China;Experiment Teaching Center for Chemistry and Chemical Engineering, Bijie University, Bijie 551700, China;Key Laboratory on Applied Chemistry, Bijie 551700, China |
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| Abstract: | |
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| Keywords: | gastrodine uridine guanosine adenosin HPLC |
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