硝菔通结方对功能性便秘大鼠排便及胃肠动力的影响

目的:探讨硝菔通结方对功能性便秘大鼠排便作用及胃肠动力相关指标的影响。方法:成年SD雄性大鼠50只,随机分为空白对照组、模型组、硝菔通结方高、中、低剂量组(380,190,95 g·kg-1)各10只。采用复方地芬诺酯(15 mg·kg-1)灌胃4周,建立SD大鼠功能性便秘模型,造模成功后治疗组给予硝菔通结方灌胃3周。观察各组大鼠体重、食量、粪便量和一般行为学表现,Power Lab检测大鼠在体结肠肌电,ELISA检测大鼠血清中胃动素、胃泌素的含量。结果:与空白组比较,模型组大鼠体重减轻,摄食量、粪便量均减少,有显著性差异(P<0.01);与模型组比较,硝菔通结方高、中剂量组大鼠体重、摄食量及大便量均增加,尤以高剂量组最为明显(P<0.01)。较空白组,模型组大鼠在体结肠肌电慢波频率减慢,振幅增大;与模型组比较,硝菔通结方各剂量组频率加快,振幅减低,尤以高剂量组效果最为明显(P<0.01)。与空白组比较,模型组大鼠血清中胃动素、胃泌素含量降低;与模型组比较,硝菔通结方各剂量组大鼠血清中胃动素、胃泌素含量升高,尤以高剂量组最为明显(P<0.01)。结论:硝菔通结方可通过改善便秘大鼠的便秘症状及大鼠胃肠动力起到治疗功能性便秘的作用。

Effect of Xiaofu Tongjie Fang on Functional Constipation Rats Defecation Function and Gastrointestinal Motility

Objective: To explore the effect of Xiaofu Tongjie fang on functional constipation defecation function and gastrointestinal motility index. Method: Fifty adult male SD rats were randomly divided into blank control group, model group, Xiaofu Tongjie fang high dose group,Xiaofu Tongjie fang medium dose group, Xiaofu Tongjie fang low dose group with ten rats in each group. SD rats with functional constipation model were estabilished by oral administration of compound diphenoxylate tablets for 4 weeks. After the success of modeling, the treatment group was given Xiaofu Tongjie fang for 3 weeks. Performance of different groups of rats, food intake, feces weightand general behavior. Rats' colonic electromyography was detected by Power Lab. The serum contents of motilin and gastrin were detected by ELISA. Result: Compared with the control group, in the model group the body weight, food intake, feces quantity were decreased, with significant difference (P<0.01). Compared with the model group, the body weight, food intake and fecal quantity of rats were increased in Xiaofu Tongjie fang high and medium dose group, which were markly increased in the high dose group(P<0.01). Compared with the blank group, the frequency of in vivo colonic electromyography slow wave was decreasedand the amplitudewas increased, in all groups of Xiaofu Tongjie fang, which were markly changed in the high dose group (P<0.01). The serum contents of motilin and gastrin in all groups of Xiaofu Tongjie fang group were decreased as compared with the blank group, and which were increased markly for high dose group as compared with the control group(P<0.01). Conclusion: Xiaofu Tongjie fang can treat functional contipation by impoving the symptoms and the gastrointestinal motility in functional constipation rats.