丹参水提液的纳滤浓缩工艺考察

目的:摸索丹参水提液的纳滤浓缩工艺。方法:选择热不稳定成分丹酚酸B为检测对象,采用HPLC测定丹酚酸B保留率,检测波长281 nm,流动相乙腈(A)和1%冰乙酸(B)梯度洗脱(0~20 min,5%~15%A;20~50 min,15%~40%A)。以丹酚酸B保留率和膜通量为指标,通过单因素试验分析截留相对分子质量、操作压力、吸附特征等因素对丹参水提液浓缩工艺的影响。结果:丹酚酸B的保留率随着膜相对分子质量减小而增大,受压力、浓缩倍数的影响较小;膜通量与压力、截留相对分子质量呈正相关,随着浓缩倍数的增大而减小;孔径300 Da纳滤膜对丹酚酸B的保留率99.5%,膜通量12 L·m-2·h-1,对丹参药材中其他成分无明显影响,且浓缩效率高。结论:纳滤浓缩适用于含热不稳定类成分的药液浓缩,效率高且成分损失少。

Analysis of Nanofiltration Enrichment Process of Extract Solution of Salviae Miltiorrhizae Radix et Rhizoma

Objective: To find out nanofiltration enrichment process of extract solution of Salviae Miltiorrhizae Radix et Rhizoma. Method: Taking salvianolic acid B as detection object,HPLC was employed to determine retention rate of salvianolic acid B with mobile phase of acetonitrile(A)-1% acetic acid(B) for gradient elution(0-20 min,5%-15%A;20-50 min,15%-40%A) and detection wavelength at 281 nm.With retention rate and membrane flux of salvianolic acid B as indexes,effects of operating pressure,adsorption characteristics and other factors on concentration process were investigated by single factor tests. Result: Retention rate of salvianolic acid B increased with decreasing of aperture and unchanged with change of pressure and cycles of concentratin.Membrane flux was positively correlated with intercept relative molecular weight and pressure,decreased as concentration ratio increased.Retention rate of salvianolic acid B was 99.5% when nanofiltration membranes aperture was 300 Da,membrane flux was 12 L·m^-2·h^-1,this process had no obvious influence to other ingredients in Salviae Miltiorrhizae Radix et Rhizoma with high concentration efficiency. Conclusion: Nanofiltration concentration is useful for concentrating liquid including thermal instability class ingredients,it has high efficiency and low losses of active ingredients.