草麻黄抗补体组份对血小板变形及内皮细胞损伤作用

目的从草麻黄中分离抗补体组份并观察其抗补体依赖性的损伤作用。方法采用柱层析结合紫外吸收观察的方法分离抗补体组份；采用比浊法观察组份对眼镜蛇毒因子引起的血小板变形的影响；采用MTT法观察组份对异种血清对内皮细胞损伤的影响。结果紫外吸收为196nm的组份具有抗补体作用，麻黄组份能有效抑制眼镜蛇毒因子引起的血小板变形，高、中、低剂量对血小板变形的抑制率分别为49．8％±10．7％、32．8％±4．2％、22．5％±6．2％；麻黄组份能有效减轻异种血清对内皮细胞的损伤，高、中、低剂量对补体致内皮细胞损伤抑制率分别为88．1％±7．1％、79．8％±3．3％、65．0％±4．5％。结论麻黄抗补体组份对补体依赖性损伤有保护作用。

Isolation of Anti-complement Components from Ephedra Sinica andits Protective Effect on Complement-dependent Injury

ObjectiveTo isolate the anti-complement components from ephedra sinica and investigate its protective effect on complement-dependent injury.MethodsAnti-complement components were seprerated by using column chromatography;turbidimetric assay was used to investigate the effect of anti-complement component on CVF-induced platelet metamorphose;MTT method was used to investigate the effect of anti-complement components on endothelial cells injury caused by rabbit serum.ResultsAnti-complement components effectively reduced CVF-induced platelet metamorphose and attenuated the endothelial cells injury caused by rabbit serum.ConclusionAnti-complement components may have protective effect on complement-dependent injury.