蓝舌病毒两外壳蛋白VP2和VP5在昆虫细胞中的表达 …

目的 研究蓝舌病毒（ＢＴＶ）ＶＰ２与ＶＰ５的免疫学特性，为ＢＴＶ基因工程疫苗研究和病毒样颗粒装配打下基础。方法 将ＢＴＶ１０ ＶＰ２和ＶＰ５基因分别插入杆状病毒表达载体ｐＦａｓｔＢａｃ１，转染昆虫细胞获得重组杆状病毒。用ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎ ｂｌｏｔ检测重组杆状病毒对ＶＰ２和ＶＰ５的表达，运用组织培养中和试验和直接血凝试验检测表达产物的生物学活性。

Expression and characterization of two outer capsid proteins VP2 and VP5 of bluetongue virus in insect cells

OBJECTIVE: To study the biological characteristics of the outer capsid proteins VP2 and VP5 of bluetongue virus (BTV) expressed in insect cells and their potential use in the assembly of BTV and genetic engineering vaccine. METHODS: The genes which encode the two outer capsid proteins VP2 and VP5 of bluetongue virus (BTV) 10 were separately cloned into pFastBac1 vector and the corresponding recombinant baculoviruses were obtained. RESULTS: BTV VP2 could be expressed in Sf-9 cells better than VP5. Further works indicated that VP2 could elicit neutralizing antibodies to BTV10(1:64), and also could partially neutralize BTV1(1:16), but could not neutralize BTV13. The co-immunizing of VP2 and VP5 could induce higher neutralizing antibodies to BTV10 and BTV1. VP2 also showed a hemagglutination activity. CONCLUSION: VP2 expressed in insect cells could induce neutralizing antibodies to BTV and had the biological activity of hemagglutination, VP5 could enhance the ability of neutralizing antibody induction of VP2, they can be used for the assembly of virus-like particles and for the development of genetic engineering vaccine.