先天性心脏病心肌活检组织中微小病毒感染的定位

目的 了解人微小病毒Ｂ１９（Ｐａｖｏｖｉｎｕｓ Ｂ１９）感染在先天性心脏病（Ｃｏｎｇｅｎｔｉａｌ ｈｅａｒｔ ｄｉｓｅａｓｅ，ＣＨＤ）心脏组织中的定位。方法 采用巢式聚合酶链反应（ＰＣＲ）和组织原位杂交（ＩＳＨ）技术，对３７例ＣＨＤ患者外周血及手术活检的心肌组织和２８例非先天畸形心肌组织进行Ｂ１９ ＤＮＡ的检测，结果 ３７例ＣＨＤ组中Ｂ１９ ＤＮＡ在血清及心肌组织中阳性率分别为１６．２２％（６／３

The localization of parvovirus B19 in cardiac biopsy tissue of congenital heart disease

Objective To explore the relationship between parvovirus B19 infection and congenital heart disease (CHD), and distribution of parvovirus B19 gene in cardiac tissue.Methods We conducted a case controls study invesigaing the presence of B19 gene in cardiac biopsy tissue of 37 cases of CHD, 8 cases of rheumatic heart disease(RHD) and 20 cases of non CHD with nested polymerase chain reaction(Nested PCR) and in situ hybridization(ISH).Results Among 37 CHD patients, 6 were B19 DNA positive in cardiac tissue (18 92%), while in control group, including 8 RHD patients (autopsy specimens) and 20 non CHD patients, the B19 gene were all negative, there was a significant difference of B19 gene presence between CHD group and control group (P=0 0421). ISH of B19 gene in cardiac tissue were positive in 4 CHD patients and all were negative in control group. The B19 gene were found locating in the nucleus of cardiac cell by ISH.Conclusion Parvovirus B19 infection correlated with CHD.Since B19 gene was mainly found in the cardiac cell nucleus, maybe it influenced the regulation of gene expression and affected the development of heart with the reuslt of CHD.