| IgG分子结构变化增强静注免疫球蛋白抗补体活性(英文) |
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| 引用本文： | 臧星星,孙艳,沈斐,王东,周雷,王坚,龚鹏. IgG分子结构变化增强静注免疫球蛋白抗补体活性(英文)[J]. Acta pharmacologica Sinica, 1995, 0(5) |
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| 作者姓名： | 臧星星 孙艳 沈斐 王东 周雷 王坚 龚鹏 |
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| 作者单位： | 中美RAAS血制品有限公司质量控制部，中美RAAS血制品有限公司质量控制部，中美RAAS血制品有限公司质量控制部，中美RAAS血制品有限公司质量控制部，中美RAAS血制品有限公司质量控制部，中美RAAS血制品有限公司质量控制部，中美RAAS血制品有限公司质量控制部 上海 200240 中国 Department of Basic Immunology，Shanghai Institute of Immunology (WHO Immunology Collaboration Center)，Shanghai Second Medical University，Shanghai 200025，China.，上海 200240 中国，上海 200240 中国，上海 200240 中国，上海 200240 中国，上海 200240 中国，上海 200240 中国 |
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| 摘 要： | 目的:研究静注免疫球蛋白(IVIG)产生抗补体活性(ACA)的原因.方法:用二种方法测定ACA活性,高效液相色谱测定IgG多聚体、双聚体、单体及碎片组成,环状免疫扩散法测定IgG亚类和IgA含量,显色反应测定前激肽释放酶激活因子(PKA)和激肽释放酶(KK)活性.酶联免疫吸附试验测定针对乙肝表面抗原的特异抗体(Anti-HBs).结果:二种ACA方法有相关性,但ACA活性与各种IgG分子组成、IgG亚类和IgA含量、PKA和KK无关.IVIG热培养后ACA活性显著升高,Anti-HBs活性则明显下降,而各种IgG分子组成则基本未变.结论:IgG分子结构变化增强IVIG自发抗补体活性.
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| 关 键 词： | 静脉注射免疫球蛋白 补体激活 免疫球蛋白G 前激肽释放酶 激肽释放酶 多聚体 |
Molecular structure alteration of IgG increased anticomplementary activity of intravenous immunoglobulin |
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| ZANG Xing-Xing,SUN Yan,SHEN Fei,WANG Dong,ZHOU Lei,WANG Jian,Eric KUNG. Molecular structure alteration of IgG increased anticomplementary activity of intravenous immunoglobulin[J]. Acta pharmacologica Sinica, 1995, 0(5) |
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| Authors: | ZANG Xing-Xing SUN Yan SHEN Fei WANG Dong ZHOU Lei WANG Jian Eric KUNG |
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| Abstract: | AIM:To study the cause of anticomple-mentary activities (ACA) of intravenous im-munoglobulins (IVIG). METHODS, ACA were determined by two assays (limit test and 100CH50 test), distributions of IgG molecular size (polymer,dimer,monomer and fragment) by HPLC, IgG subclasses and IgA contents by radial immunodiffusion, prekallikrein activator (PKA) and kallikrein (KK) activities by chromogenic assay, potency of antibody against hepatitis B surface antigen (Anti-HBs) by enzyme-linked immunosorbent assay (ELISA). RESULTS: The two ACA assays showed good correlation. However, ACA levels were not associated with distributions of IgG molecular size, IgG subclasses and IgA contents, PKA and KK activities. After heating incubation, ACA levels increased markedly and Anti-HBs decreased notably, distributions of IgG molecular size remained relatively constant. CONCLUSION: Molecular structure alteration of IgG increased spontaneous complement activation of IVIG. |
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| Keywords: | intravenous immunoglobulins complement activation IgG prekallikrein kallikrein polymer |
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