海洛因对大鼠小肠腺苷酸脱氨酶活性的影响

目的：通过检测大鼠小肠腺苷脱氨酶（ADA）和血浆尿酸的含量探讨海洛因对大鼠小肠腺苷酸分解代谢的影响。方法：建立海洛因给药、停药大鼠模型。腹主动脉取血，测定血浆尿酸含量；处死后取小肠组织制备10%组织匀浆，测ADA活性。结果：海洛因给药9 d组与对照组比较大鼠血浆尿酸水平明显升高（P<0.01），小肠组织ADA活性增高（P<0.05）；自然停药8 d组与海洛因给药9 d组比较，小肠组织中ADA明显降低（P<0.05），尿酸水平降低，但差异无显著性（P>0.05）。结论：海洛因通过增加ADA的活性，使小肠组织嘌呤核苷酸分解代谢增强。

Effect of heroin on activity of adenosine deaminase in rat small intestine

Objective To study the effects of heroin on adenosine deaminase (ADA) in small inteseine and plasma uric acid content in rats. Methods The rat models injected with heroin and forbidden to heroin were set up. The blood samples from the abdominal aorta were obtained, then the plasma uric acid content was detected. 10% homogenate of small intestine was prepared after the rats were killed, then the ADA activity was determined. Results Compared with the control group, the content of plasma uric acid of the rats increased significantly after administration of heroin for 9 d (P<0. 01), the ADA activity in small intestine increased (P<0. 05). Compared with 9 d heroin administration group, the content of plasma uric acid in 8 d heroin withdrawal group decreased, but the difference was not significant (P>0. 05); and the ADA activity in small intestine decreased significantly (P< 0. 05). Conclusion The results may suggest that long-term heroin administration can enhance the catabolism of purine nucleotide in small insestine by increasing the activity of ADA.