利用FISH分析HPV16在Hela细胞中的整合位点

 目的 探讨HPV16病毒感染导致子宫颈癌发生的染色体畸变。方法 利用荧光原位杂交（FISH）技术检测HPV16病毒感染导致的Hela细胞中染色体核型的变化。结果 Hela细胞系中存在9号染色体的三体畸变，但3号染色体表现为正常的二倍体核型；Hela细胞中没有发现HPV16的整合位点，但当外源性的HPV16感染Hela细胞后，HPV16病毒DNA整合在3号染色体上；9号和3号染色体变异在子宫颈癌的发生发展中起着重要的作用，而HPV16的感染主要引起3号染色体的变异。结论 HPV16可以引起Hela细胞的3号染色体的畸变，从而导致子宫颈癌的发生。

Mapping and analysis of HPV16 integration sites in a cervical cancer cell line

Objective To explore the chromosome aberration of cervical cancers induced by human papillomavirus (HPV16) virus. Methods The change of change of chromosomes of Hela cells induced by HPV-16 infection HPV-16 in fection was detected by FISH. Results The integration sites of HPV-16 were not observed in Hela cells. But HPV16 virus was integrated on chromosomes 3. Conclusion HPV16 could cause chromosome 3 aberration in Hela cells and result in the occurance of cervical cancers