温阳活血方对慢性马兜铃酸肾病大鼠肾损害的干预作用

目的：观察温阳活血方对慢性马兜铃酸肾病肾损害的干预作用。方法：将48只雄性SD大鼠随机分为5组：（1）正常对照组（n=8）：予生理盐水灌胃；（2）模型组（n=10）：按关木通水煎液10ml·kg^-1·d^-1（相当于关木通40g·kg^-1·d^-1，马兜铃酸A2．6mg·kg^-1·d^-1）给大鼠灌胃；（3）中药组（n=10）：在模型组基础上，再予温阳活血方30g·kg^-1·d^-1灌胃；（4）西药组（n=10）：在模型组基础上，再予科素亚33．3mg·kg^-1·d^-1灌胃；（5）中西药结合组（n=10）：在模型组基础上，再予温阳活血方＋科素亚灌胃。20周末，收集大鼠尿液测定24h尿蛋白、NAG、β2-MG，腹主动脉取血用于测定Scr、BUN、RBC、Hb。结果：与正常对照组比较，模型组大鼠24h尿蛋白、NAG、β2-MG、Scr、BUN均明显升高（P〈0．01，P〈0．05），而血Hb、RBC均明显下降（P〈0．01）；与模型组比较，治疗组大鼠24h尿蛋白、NAG、β2-MG、Scr、BUN均明显下降（P〈0．01，P〈0．05），而血RBC、Hb均明显升高（P〈0．01，P〈0．05）。结论：温阳活血方对慢性马兜铃酸肾病大鼠肾损害有一定的保护作用，能降低尿蛋白和尿NAG、β2-MG的排泄，改善肾功能和贫血。

Intervention of Wen Yang Huo Xue Fang on Kidney Damage in Rats with Chronic Aristolochic Acid Nephropathy

Objective：To study the effects of Wen Yang Huo Xue Fang（WYHXF） on urine protein, renal function, Hb and RBC in rats with chronic aristolochic acid nephropathy. Methods： Fourty - eight male SD rats were randomly divided into five groups. C-roup A（ n = 8） were treated with normal .saline（ 10 ml·kg^-1·d^-1 ）. Group B（ n = 10） were treated with caulis Aristolochia Manshuriensis decoction（Aristolochia Manshuriensis 40 g·kg^-1·d^-1, Aristolochic acid A2.6 mg·kg^-1·d^-1 ） to establishe the model. Group C（ n= 10） were treated with WYHXF（30 g·kg^-1·d^-1 ） after the rats model established with the above-mentioned method. Group D（n =10） were treated with Losartan Potassium Tablets （33.3 mg·kg^-1·d^-1） after the rat model established with the above - mentioned method. Group E（ n - 10） were treated with WYHXF（ 30 g·kg^-1·d^-1 ） and Losartan Potassium Tablets （ 33.3 mg·kg^-1·d^-1） after the rat model established with the above - mentioned method. After 20 weeks, rats＇ urine was collected for the detection of 24 hour urine protein, NAG and β2 - MG. Blood from abdominal aortic artery was sampled for the detection of Scr, BUN, RBC and Hb. Results：Compared with those in the group A,the levels of 24 - hour urine protein, NAG,β2 - MG,Scr and BUN were significantly increased in the group B （P〈 0.01, P〈 0.05）, while the level of RBC and Hb were significantly decreased in the group B（ P〈 0.01 ）. Compared with those in the group B, the levels of 24 - hour urine protein, NAG, β2 - MG, Scr and BUN were significantly decreased（ P 〈 0.01, P 〈 0.05 ）, while the levels of RBC and Hb were significantly increased in the group C, D and E （P 〈 0.01, P 〈 0.05）. Conclusion： WYHXF could reduce the levels of urine protein, NAG and β2 - MG and improve renal function and alleviate anemia in chronic aristolochie acid nephropathy.